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Molecular structure and spatial expression of a homeobox gene from the labial region of the Antennapedia-complex.

机译:来自触角复合体唇部区域的同源异型框基因的分子结构和空间表达。

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摘要

We report the molecular characterization of a homeobox-containing gene that maps at 84A in the proximal region of the Antennapedia-complex. The structure and complete sequence are presented. Deletion analysis indicates that the cloned gene, F24, most likely corresponds to the labial (lab) gene. Northern blot experiments show a single approximately 3-kb transcript that is expressed at all embryonic stages from cellular blastoderm onwards and during larval development. The homeobox is split by an intron in the region which encodes the putative DNA-binding helix, a splicing position for homeobox-containing genes which is unique so far. The 5' part of the gene contains four M-repeat sequences (CAA/G repeats) in the protein-coding region. In situ hybridization to the transcripts during embryogenesis reveals two domains of expression. The anterior one is located in parts of the developing head, mainly in the hypopharyngeal organ and in anterior parts of the mandibular lobe, and is restricted to the ectoderm. The posterior domain is part of the posterior midgut primordium (endoderm), that invaginates and later contacts the endoderm cells from the anterior midgut invagination.
机译:我们报告了映射在Antennapedia-complex的近端区域中在84A处包含一个同源框的基因的分子表征。介绍了结构和完整序列。缺失分析表明,克隆的基因F24最有可能对应于阴唇(lab)基因。 Northern印迹实验显示,从细胞胚盘开始到幼虫发育的所有胚胎阶段均表达约3kb的转录本。同源盒在编码推定的DNA结合螺旋的区域中被内含子分裂,到目前为止,该序列是含同源盒的基因的剪接位置。基因的5'部分在蛋白质编码区域包含四个M重复序列(CAA / G重复)。胚胎发生过程中与转录本的原位杂交揭示了两个表达域。前者位于发育头部的某些部位,主要位于下咽器官和下颌叶的前部,并局限于外胚层。后结构域是后肠中原基(内胚层)的一部分,其从前中肠内陷侵入并随后接触内胚层细胞。

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