• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>The EMBO Journal >Lrp a major regulatory protein in Escherichia coli bends DNA and can organize the assembly of a higher-order nucleoprotein structure.
【2h】

Lrp a major regulatory protein in Escherichia coli bends DNA and can organize the assembly of a higher-order nucleoprotein structure.

机译：Lrp是大肠杆菌中的主要调节蛋白可使DNA弯曲并可以组织更高阶核蛋白结构的组装。

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Lrp (Leucine-responsive regulatory protein) is a global regulatory protein that controls the expression of many operons in Escherichia coli. One of those operons, ilvIH, contains six Lrp binding sites located within a several hundred base pair region upstream of the promoter region. Analysis of the binding of Lrp to a set of circularly permuted DNA fragments from this region indicates that Lrp induces DNA bending. The results of DNase I footprinting experiments suggest that Lrp binding to this region facilitates the formation of a higher-order nucleoprotein structure. To define more precisely the degree of bending associated with Lrp binding, one or two binding sites were separately cloned into a pBend vector and analyzed. Lrp induced a bend of approximately 52 degrees upon binding to a single binding site, and the angle of bending is increased to at least 135 degrees when Lrp binds to two adjacent sites. Lrp-induced DNA bending, and a natural sequence-directed bend that exists within ilvIH DNA, may be architectural elements that facilitate the assembly of a nucleoprotein complex.
机译：Lrp（亮氨酸反应性调节蛋白）是一种全球调节蛋白，可控制大肠杆菌中许多操纵子的表达。这些操纵子之一，ilvIH，包含六个Lrp结合位点，位于启动子区域上游几百个碱基对区域内。 Lrp与来自该区域的一组圆形排列的DNA片段的结合分析表明，Lrp诱导DNA弯曲。 DNase I足迹实验的结果表明，Lrp与该区域的结合有助于形成更高阶的核蛋白结构。为了更精确地定义与Lrp结合相关的弯曲程度，将一个或两个结合位点分别克隆到pBend载体中并进行分析。 Lrp与单个结合位点结合后会引起大约52度的弯曲，当Lrp与两个相邻位点结合时，弯曲角度会增加到至少135度。 Lrp诱导的DNA弯曲和ilvIH DNA中存在的天然序列定向弯曲可能是促进核蛋白复合物组装的结构元件。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号