首页> 美国卫生研究院文献>The EMBO Journal >The mating-type locus B alpha 1 of Schizophyllum commune contains a pheromone receptor gene and putative pheromone genes.
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The mating-type locus B alpha 1 of Schizophyllum commune contains a pheromone receptor gene and putative pheromone genes.

机译:Schizophyllum commune的交配型基因座B alpha 1包含信息素受体基因和推定的信息素基因。

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摘要

Analysis of the multispecific B alpha mating-type locus of Schizophyllum commune provided evidence that pheromones and pheromone receptors govern recognition of self versus non-self and sexual development in this homobasidiomycetous fungus. Four subclones of an 8.2 kb genomic fragment carrying B alpha 1 specificity induced B-regulated sexual morphogenesis when introduced into a strain with one of the eight compatible B alpha specificities that are known to exist in nature. One of these clones, which activated all other B alpha specificities, contains a gene termed bar1. The predicted protein product of bar1, as well as that of bar2, a homologous gene isolated from a B alpha 2 strain, has significant homology to known fungal pheromone receptor proteins in the rhodopsin-like superfamily of G protein-linked receptors. The other three active B alpha 1 clones were subcloned further to identify the minimal active element in each clone. Every active subclone contains a putative pheromone gene ending in a signal for possible isoprenylation. A message of approximately 600 bp was observed for one of these genes, bap1(1). This paper presents the first evidence for a system of multiple pheromones and pheromone receptors as a basis for multispecific mating types in a fungus.
机译:对Schizophyllum Commun的多特异性B alpha交配型基因座的分析提供了证据,表明信息素和信息素受体支配了该同种担子菌真菌对自身和非自身以及性发育的识别。当将带有B alpha 1特异性的8.2 kb基因组片段的四个亚克隆引入已知自然界中存在的八种兼容B alpha特异性之一的菌株时,会诱导B调节性形态发生。这些可激活所有其他B alpha特异性的克隆之一包含一个名为bar1的基因。 bar1的预测蛋白质产物以及bar2的预测蛋白质产物,是从B alpha 2株中分离的同源基因,与G蛋白连接受体的视紫红质样超家族中的已知真菌信息素受体蛋白具有显着同源性。将其他三个活性B alpha 1克隆进一步亚克隆,以鉴定每个克隆中的最小活性元素。每个活跃的亚克隆都包含一个推定的信息素基因,该信息素以可能的异戊二烯化信号结束。这些基因之一bap1(1)的消息大约为600 bp。本文为多种信息素和信息素受体系统提供了第一个证据,作为真菌中多种特异性交配类型的基础。

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