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首页> 美国卫生研究院文献>The EMBO Journal >Role of a white collar-1-white collar-2 complex in blue-light signal transduction.
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Role of a white collar-1-white collar-2 complex in blue-light signal transduction.

机译：白领1白领2复合体在蓝光信号传导中的作用。

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摘要

Mutations in either white collar-1 (wc-1) or white collar-2 (wc-2) lead to a loss of most blue-light-induced phenomena in Neurospora crassa. Sequence analysis and in vitro experiments show that WC-1 and WC-2 are transcription factors regulating the expression of light-induced genes. The WC proteins form homo- and heterodimers in vitro; this interaction could represent a fundamental step in the control of their activity. We demonstrate in vivo that the WC proteins are assembled in a white collar complex (WCC) and that WC-1 undergoes a change in mobility due to light-induced phosphorylation events. The phosphorylation level increases progressively upon light exposure, producing a hyperphosphorylated form that is degraded and apparently replaced in the complex by a newly synthesized WC-1. WC-2 is unmodified and also does not change quantitatively in the time frame examined. Light-dependent phosphorylation of WC-1 also occurs in a wc-2 mutant, suggesting that a functional WC-2 is dispensable for this light-specific event. These results suggest that light-induced phosphorylation and degradation of WC-1 could play a role in the transient expression of blue-light-regulated genes. Our findings suggest a mechanism by which WC-1 and WC-2 mediate light responses in Neurospora.
机译：白领1（wc-1）或白领2（wc-2）中的突变会导致crus Neurospora crassa中大多数由蓝光引起的现象的丧失。序列分析和体外实验表明，WC-1和WC-2是调控光诱导基因表达的转录因子。 WC蛋白在体外形成同型和异型二聚体。这种互动可能是控制其活动的基本步骤。我们在体内证明了WC蛋白在白领复合体（WCC）中组装，并且WC-1由于光诱导的磷酸化事件而经历了迁移性变化。磷酸化水平在曝光后会逐渐增加，从而产生一种高磷酸化形式，该形式在复合物中被降解并明显被新合成的WC-1取代。 WC-2未修改，在检查的时间范围内也没有定量变化。 WC-1的光依赖性磷酸化也发生在wc-2突变体中，表明功能性WC-2对于此光特异性事件是可有可无的。这些结果表明，光诱导的WC-1的磷酸化和降解可能在蓝光调节基因的瞬时表达中起作用。我们的发现提示了WC-1和WC-2介导Neurospora中光反应的机制。

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