• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>The EMBO Journal >A small protein that mediates the activation of a two-component system by another two-component system
【2h】

A small protein that mediates the activation of a two-component system by another two-component system

机译：一种小蛋白质介导另一种双组分系统激活一种双组分系统

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The PmrA–PmrB two-component system of Salmonella enterica controls resistance to the peptide antibiotic polymyxin B and to several antimicrobial proteins from human neutrophils. Transcription of PmrA-activated genes is induced by high iron, but can also be promoted by growth in low magnesium in a process that requires another two-component system, PhoP–PhoQ. Here, we define the genetic basis for the interaction between the PhoP–PhoQ and PmrA–PmrB systems. We have identified pmrD as a PhoP-activated gene that mediates the transcriptional activation of PmrA-regulated genes during growth in low magnesium. When transcription of pmrD is driven from a heterologous promoter, expression of PmrA-activated genes occurs even at repressing magnesium concentrations and becomes independent of the phoP and phoQ genes. The PmrD effect is specific for PmrA-regulated genes and requires functional PmrA and PmrB proteins. A pmrD mutant is sensitive to polymyxin if grown in low magnesium, but resistant if grown in high iron. The PmrD protein controls the activity of the PmrA–PmrB system at a post-transcriptional level.
机译：肠沙门氏菌的PmrA–PmrB两组分系统可控制对肽类抗生素多粘菌素B和人类嗜中性粒细胞的几种抗菌蛋白的抗性。 PmrA激活基因的转录是由高铁诱导的，但是在需要另一种两组分系统PhoP–PhoQ的过程中，低镁的生长也可以促进转录。在这里，我们定义了PhoP–PhoQ与PmrA–PmrB系统之间相互作用的遗传基础。我们已经确定pmrD是PhoP激活的基因，可在低镁生长期间介导PmrA调节的基因的转录激活。当pmrD的转录由异源启动子驱动时，PmrA激活基因的表达即使在抑制镁浓度下也发生，并且独立于phoP和phoQ基因。 PmrD效应对PmrA调控的基因特有，需要功能性PmrA和PmrB蛋白。如果在低镁条件下生长，pmrD突变体对多粘菌素敏感，而在高铁条件下生长则具有抗性。 PmrD蛋白在转录后水平上控制PmrA–PmrB系统的活性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号