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首页> 美国卫生研究院文献>Environmental Health Perspectives >A comparison of the reproductive physiology of largemouth bass Micropterus salmoides collected from the Escambia and Blackwater Rivers in Florida.
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A comparison of the reproductive physiology of largemouth bass Micropterus salmoides collected from the Escambia and Blackwater Rivers in Florida.

机译：从佛罗里达州的埃斯坎比亚河和黑水河收集的大嘴鲈小鳍鲈的生殖生理比较。

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摘要

Largemouth bass (LMB), Micropterus salmoides, were taken from the Escambia River (contaminated site) and the Blackwater River (reference site) near Pensacola, Florida. The Escambia River collection occurred downstream of the effluent from two identified point sources of pollution. These point sources included a coal-fired electric power plant and a chemical company. Conversely, the Blackwater River's headwaters and most of its length flow within a state park. Although there is some development on the lower part of the Blackwater River, fish were collected in the more pristine upper regions. Fish were captured by electroshocking and were maintained in aerated coolers. Physical measurements were obtained, blood was taken, and liver and gonads were removed. LMB plasma was assayed for the concentration of 17ss-estradiol (E2) and testosterone using validated radioimmunoassays. The presence of vitellogenin was determined by gel electrophoresis (SDS-PAGE) and Western blotting using a monoclonal antibody validated for largemouth bass vitellogenin. No differences in plasma concentrations of E2 or testosterone were observed in females from the two sites. Similarly, males exhibited no difference in plasma E2. However, plasma testosterone was lower in the males from the contaminated site, as compared to the reference site. Vitellogenic males occurred only at the contaminated site. Additionally, liver mass was proportionately higher in males from the contaminated site, as compared to males from the reference site. These data suggest that reproductive steroid levels may have been altered by increased hepatic enzyme activity, and the presence of vitellogenic males indicates that an exogenous source of estrogen was present in the Escambia River.
机译：大嘴鲈（LMB），Micropterus salmoides，取自佛罗里达州彭萨科拉附近的埃斯坎比亚河（受污染的地点）和黑水河（参考地点）。埃斯坎比亚河的收集发生在废水的下游，来自两个确定的污染源。这些点源包括燃煤发电厂和一家化学公司。相反，黑水河的源头和大部分长度在州立公园内流动。尽管黑水河下游有一些发展，但鱼类仍收集在较原始的上游地区。鱼被电击捕获，并保持在充气冷却器中。获得了身体测量结果，抽取了血液，并去除了肝脏和性腺。使用经过验证的放射免疫分析法检测LMB血浆中17β-雌二醇（E2）和睾丸激素的浓度。通过凝胶电泳（SDS-PAGE）和使用经大口黑鲈卵黄蛋白原验证的单克隆抗体进行蛋白质印迹法确定卵黄蛋白原的存在。在两个部位的雌性中，未观察到血浆E2或睾丸激素浓度的差异。同样，雄性在血浆E2中也没有差异。然而，与参考部位相比，受污染部位男性的血浆睾丸激素水平较低。卵黄变性雄性仅在受污染的地方发生。另外，与来自参考部位的雄性相比，来自受污染部位的雄性的肝脏质量成比例地更高。这些数据表明，肝酶活性的增加可能已改变了生殖类固醇的水平，卵黄性雄性的存在表明埃斯坎比亚河中存在外源性雌激素。

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