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首页> 美国卫生研究院文献>Environmental Health Perspectives >Biomarkers Signal Contaminant Effects on the Organs of English Sole (Parophrys vetulus) from Puget Sound
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Biomarkers Signal Contaminant Effects on the Organs of English Sole (Parophrys vetulus) from Puget Sound

机译：生物标志物对普吉特海湾英语脚底（Parophrys vetulus）器官的污染效应信号

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Fish living in contaminated environments accumulate toxic chemicals in their tissues. Biomarkers are needed to identify the resulting health effects, particularly focusing on early changes at a subcellular level. We used a suite of complementary biomarkers to signal contaminant-induced changes in the DNA structure and cellular physiology of the livers and gills of English sole (Parophrys vetulus). These sediment-dwelling fish were obtained from the industrialized lower Duwamish River (DR) in Seattle, Washington, and from Quartermaster Harbor (QMH), a relatively clean reference site in south Puget Sound. Fourier transform–infrared (FT-IR) spectroscopy, liquid chromatography/mass spectrometry (LC/MS), and gas chromatography/mass spectrometry (GC/MS) identified potentially deleterious alterations in the DNA structure of the DR fish livers and gills, compared with the QMH fish. Expression of CYP1A (a member of the cytochrome P450 multigene family of enzymes) signaled changes in the liver associated with the oxidation of organic xenobiotics, as previously found with the gill. The FT-IR models demonstrated that the liver DNA of the DR fish had a unique structure likely arising from exposure to environmental chemicals. Analysis by LC/MS and GC/MS showed higher concentrations of DNA base lesions in the liver DNA of the DR fish, suggesting that these base modifications contributed to this discrete DNA structure. A comparable analysis by LC/MS and GC/MS of base modifications provided similar results with the gill. The biomarkers described are highly promising for identifying contaminant-induced stresses in fish populations from polluted and reference sites and, in addition, for monitoring the progress of remedial actions.
机译：生活在受污染环境中的鱼类在其组织中积累了有毒化学物质。需要生物标记物来确定由此产生的健康影响，尤其是关注亚细胞水平的早期变化。我们使用了一套互补的生物标志物来指示污染物诱导的英语（Parophrys vetulus）的肝脏和g的DNA结构和细胞生理变化。这些居住在沉积物中的鱼获自华盛顿州西雅图市的工业化下游杜瓦米什河（DR），以及来自普吉特海湾南部相对较干净的参考点军需官港（QMH）。傅立叶变换红外（FT-IR）光谱，液相色谱/质谱（LC / MS）和气相色谱/质谱（GC / MS）确定了DR鱼肝和腮DNA结构的潜在有害变化与QMH鱼。 CYP1A（细胞色素P450多基因酶家族的成员）的表达表明肝脏中与有机异源生物的氧化有关的变化，如先前在g中发现的那样。 FT-IR模型表明，DR鱼的肝脏DNA具有独特的结构，可能是由于暴露于环境化学物质引起的。 LC / MS和GC / MS分析表明，DR鱼肝DNA中DNA碱基损伤的浓度较高，表明这些碱基修饰有助于这种离散的DNA结构。通过LC / MS和GC / MS对碱基修饰进行的可比分析提供了与ill相似的结果。所描述的生物标记物对于从污染和参考地点识别鱼类种群中污染物引起的胁迫具有很高的希望，此外，还可以用于监测补救措施的进展。

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