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Low sensitivity of nested PCR using Plasmodium DNA extracted from stained thick blood smears: an epidemiological retrospective study among subjects with low parasitaemia in an endemic area of the Brazilian Amazon region

机译：使用从染色浓血涂片中提取的疟原虫DNA进行巢式PCR的低敏感性：在巴西亚马逊地区流行地区低寄生虫血症受试者中的流行病学回顾性研究

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BackgroundThe success of PCR technique depends on many factors, such as high quality DNA pellets obtained from blood samples, good reagents and adequate conditions of amplification. Taking these limitations into account, a retrospective epidemiological study for malaria diagnosis was conducted in a mesoendemic area in the Brazilian Amazon.

机译：背景技术PCR技术的成功取决于许多因素，例如从血液样本中获得的高质量DNA沉淀，良好的试剂和适当的扩增条件。考虑到这些局限性，在巴西亚马逊河的一个中流行区进行了一项疟疾诊断回顾性流行病学研究。

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期刊名称 Malaria Journal
作者
Kézia KG Scopel; Cor JF Fontes; Álvaro C Nunes; Maria de Fátima Horta; Érika M Braga;
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作者单位

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年(卷),期 2004(3),-1
年度 2004
页码 8
总页数 6
原文格式 PDF
正文语种
中图分类寄生动物、寄生虫学;医学寄生虫学;
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1. Low sensitivity of nested PCR using Plasmodium DNA extracted from stained thick blood smears: an epidemiological retrospective study among subjects with low parasitaemia in an endemic area of the Brazilian Amazon region [J] . Kézia KG Scopel, Cor JF Fontes, álvaro C Nunes, Malaria Journal . 2004,第5期

机译：使用从染色浓血涂片中提取的疟原虫DNA进行巢式PCR的敏感性低：巴西亚马孙地区流行地区低寄生虫血症受试者的流行病学回顾性研究
2. Nucleotide Sequences of Plasmodium vivax A-Type rDNA ITS1, 5.8S, and ITS2. Elaboration of Retrospective PCR Diagnostics of Malaria Using Stained Thick Blood Smears [J] . N.V.Ivanova, E.N.Morozov, I.V.Kukina, Molecular Biology . 2001,第3期

机译：间日疟原虫A型rDNA ITS1、5.8S和ITS2的核苷酸序列。使用染色浓血涂片对疟疾进行回顾性PCR诊断的详细阐述
3. Sensitivity of nested-PCR for plasmodium detection in pooled whole blood samples and its usefulness to blood donor screening in endemic areas. [J] . Daniel Roberto Coradi de Freitas, Luciano Teixeira Gomes, Cor Jesus F Fontes, Transfusion and apheresis science: official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis . 2014,第2期

机译：巢式PCR对合并全血样品中疟原虫检测的敏感性及其对流行地区献血者筛查的有用性。
4. Comparison of Blood Smear Antigen Detection and Nested-PCR Methods for Screening Refugees from Regions Where Malaria Is Endemic after a Malaria Outbreak in Quebec Canada [O] . Momar Ndao, Etienne Bandyayera, Evelyne Kokoskin, 2004

机译：加拿大魁北克省疟疾暴发后疟疾流行地区的血液涂片抗原检测和巢式PCR方法的比较
5. Low sensitivity of nested PCR using Plasmodium DNA extracted from stained thick blood smears: an epidemiological retrospective study among subjects with low parasitaemia in an endemic area of the Brazilian Amazon region [O] . Scopel Kézia KG, Fontes Cor JF, Nunes Álvaro C, 2004

机译：使用从染色浓血涂片中提取的疟原虫DNA进行巢式PCR的敏感性低：巴西亚马孙地区流行地区低寄生虫血症受试者的流行病学回顾性研究

Low sensitivity of nested PCR using Plasmodium DNA extracted from stained thick blood smears: an epidemiological retrospective study among subjects with low parasitaemia in an endemic area of the Brazilian Amazon region

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