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Use of Bead-Based Serologic Assay to Evaluate Chikungunya Virus Epidemic Haiti

机译:基于珠的血清学检测在海地基孔肯雅病毒流行病评估中的应用

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摘要

The index case of chikungunya virus (CHIKV) in Haiti was reported during early 2014; the vector, the pervasive Aedes aegypti mosquito, promoted rapid spread throughout the country. During December 2014–February 2015, we collected blood samples from 4,438 persons at 154 sites (62 urban, 92 rural) throughout Haiti and measured CHIKV IgG by using a multiplex bead assay. Overall CHIKV seroprevalence was 57.9%; differences between rural (mean 44.9%) and urban (mean 78.4%) areas were pronounced. Logistic modeling identified the urban environment as a strong predictor of CHIKV exposure (adjusted odds ratio 3.34, 95% CI 2.38–4.69), and geographic elevation provided a strong negative correlation. We observed no correlation between age and antibody positivity or titer. Our findings demonstrated through serologic testing the recent and rapid dissemination of the arbovirus throughout the country. These results show the utility of serologic data to conduct epidemiologic studies of quickly spreading mosquitoborne arboviruses.
机译:2014年初报告了海地基孔肯雅病毒(CHIKV)的索引病例;埃及伊蚊无处不在的媒介促进了其在全国的迅速传播。在2014年12月至2015年2月期间,我们从海地的154个地点(城市中的62个,农村的92个)中的4438人采集了血液样本,并通过多重磁珠测定法测量了CHIKV IgG。 CHIKV的总体血清阳性率为57.9%;农村地区(平均44.9%)和城市地区(平均78.4%)之间的差异明显。逻辑模型确定城市环境是CHIKV暴露的强烈预测因子(调整后的优势比为3.34,95%CI为2.38-4.69),而地理高度则具有很强的负相关性。我们观察到年龄与抗体阳性或滴度之间无相关性。我们的发现通过血清学测试证明了虫媒病毒在全国各地的迅速传播。这些结果表明,血清学数据可用于快速传播蚊媒虫媒病毒的流行病学研究。

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