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A method to preserve low parasitaemia Plasmodium-infected avian blood for host and vector infectivity assays

机译:一种保存低寄生虫血症疟原虫感染的禽血的方法用于宿主和载体的感染性测定

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摘要

BackgroundAvian malaria vector competence studies are needed to understand more succinctly complex avian parasite-vector-relations. The lack of vector competence trials may be attributed to the difficulty of obtaining gametocytes for the majority of Plasmodium species and lineages. To conduct avian malaria infectivity assays for those Plasmodium spp. and lineages that are refractory to in vitro cultivation, it is necessary to obtain and preserve for short periods sufficient viable merozoites to infect naïve donor birds to be used as gametocyte donors to infect mosquitoes. Currently, there is only one described method for long-term storage of Plasmodium spp.—infected wild avian blood and it is reliable at a parasitaemia of at least 1 %. However, most naturally infected wild-caught birds have a parasitaemia of much less that 1 %. To address this problem, a method for short-term storage of infected wild avian blood with low parasitaemia (even ≤0.0005 %) has been explored and validated.
机译:背景禽类疟疾媒介能力研究需要了解更简洁的复杂禽寄生虫-媒介关系。缺乏载体能力试验的原因可能是难以获得大多数疟原虫物种和谱系的配子细胞。对那些疟原虫属进行禽类疟疾感染性测定。以及对于体外培养难以耐受的血统,有必要在短期内获得并保存足够的活裂殖子,以感染幼稚的供体鸟,用作配子体供体来感染蚊子。目前,只有一种描述的方法可以长期保存被疟原虫感染的野生禽血,并且在至少1%的寄生虫血症中是可靠的。但是,大多数自然感染的野生鸟类的寄生虫病率都远远低于1%。为了解决这个问题,已经探索并验证了一种用于短期存储低寄生虫血症(甚至≤0.0005%)的受感染野生禽血的方法。

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