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首页> 美国卫生研究院文献>Cytotechnology >Sericin a protein derived from silkworms accelerates the proliferation of several mammalian cell lines including a hybridoma
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Sericin a protein derived from silkworms accelerates the proliferation of several mammalian cell lines including a hybridoma

机译：丝胶蛋白是一种来自蚕的蛋白可加速包括杂交瘤在内的几种哺乳动物细胞的增殖

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摘要

Sericin, a constituent of the silkworm cocoon, was added to the culture of four mammalian cell lines: murine hybridoma 2E3-O,human hepatoblastoma HepG2, human epithelial HeLa and human embryonal kidney 293 cells. The proliferation of all cell lineswas accelerated in the presence of sericin. The hybridoma cellline was further studied. The 2E3-O cell line was so well adapted to serum-free medium that both the proliferation rate and maximum cell density in serum-free ASF103 medium were higher than in RPMI medium supplemented with all lots of FBS tested, and this proliferation was stimulated by the addition of sericin in a dose-dependent manner. Stimulation was observed at sericin concentrations from 0.01 to 0.1 %, although 1% sericin was severely harmful to the culture. In comparison with bovine serum albumin (BSA), a widely used supplement in serum-free medium, sericin had an equivalent effect on the proliferation of the hybridomas and sericin additively stimulated the proliferation with BSA. Although heat easily denatures and inactivates most proteins, the activity of sericin was not affected by autoclaving. In a similar manner to the silkworm-derived sericin, recombinant sericin synthesized in E. coli also stimulated the hybridoma proliferation, irrespective of whether it was autoclaved or filtered. Since BSA is obtained from bovine serum and the risk of infections such as bovine spongiform encephalopathy cannot be eradicated, sericin derived from insects could be a preferable culture medium supplement for stimulating the proliferation of mammalian cells.
机译：蚕茧的组成成分丝胶蛋白被添加到四种哺乳动物细胞系的培养中：鼠类杂交瘤2E3-O，人肝母细胞瘤HepG2，人上皮HeLa和人胚肾293细胞。在丝胶中，所有细胞系的增殖都被加速。对杂交瘤细胞系进行了进一步研究。 2E3-O细胞系非常适合无血清培养基，因此无血清ASF103培养基中的增殖速率和最大细胞密度均高于补充了所有FBS的RPMI培养基，并且这种增殖受到了以剂量依赖性方式添加丝胶。尽管1％的丝胶对培养物严重有害，但在0.01-0.1％的丝胶浓度下观察到刺激。与牛血清白蛋白（BSA）（一种在无血清培养基中广泛使用的补充剂）相比，丝胶对杂交瘤细胞的增殖具有同等作用，而丝胶与BSA共同刺激了增殖。尽管热很容易使大多数蛋白质变性并使它们失活，但是丝胶蛋白的活性不受高压灭菌的影响。与家蚕衍生的丝胶蛋白相似，在大肠杆菌中合成的重组丝胶蛋白也能刺激杂交瘤细胞的增殖，而不论其是高压灭菌还是过滤。由于牛血清白蛋白（BSA）来自牛血清，不能消除牛海绵状脑病等感染的风险，因此来源于昆虫的丝胶蛋白可能是刺激哺乳动物细胞增殖的理想培养基。

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