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Enhanced expression of gene coding for β-endorphin in human monocytic cells exposed to pulsed radio frequency electric fields through thermal and non-thermal effects

机译:通过热和非热作用在暴露于脉冲射频电场的人单核细胞中增强编码β-内啡肽的基因的表达

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摘要

BackgroundThe enhanced expression of endogenous opioid peptides, including β-endorphin, has been implicated in the mechanism of action of pulsed radio frequency (PRF) application in pain modulation. Because thermal effects cannot be separated from the physical property of PRF application to biological tissues, we evaluated whether temperatures higher than that of the normal body temperature (37°C) modulate mRNA expression for the precursor of β-endorphin, proopiomelanocortin (POMC) in human monocytic cells THP-1. We also attempted to examine whether mechanisms other than thermal effects also modulate such gene expression.
机译:背景技术内源性阿片肽(包括β-内啡肽)的增强表达与脉冲射频(PRF)在疼痛调节中的作用机制有关。由于不能将热效应与PRF应用于生物组织的物理特性分开,因此我们评估了温度是否高于正常体温(37°C)来调节β-内啡肽前体proopiomelanocortin(POMC)的mRNA表达。人单核细胞THP-1。我们还试图检查除热效应以外的其他机制是否也能调节此类基因的表达。

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