首页> 美国卫生研究院文献>Plant Methods >Development of TaqMan probes targeting the four major celiac disease epitopes found in α-gliadin sequences of spelt (Triticum aestivum ssp. spelta) and bread wheat (Triticum aestivum ssp. aestivum)
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Development of TaqMan probes targeting the four major celiac disease epitopes found in α-gliadin sequences of spelt (Triticum aestivum ssp. spelta) and bread wheat (Triticum aestivum ssp. aestivum)

机译:TaqMan探针的开发针对在拼写(小麦)和面包小麦(小麦)的α-麦醇溶蛋白序列中发现的四种主要乳糜泻抗原决定簇

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摘要

BackgroundCeliac disease (CD) is caused by specific sequences of gluten proteins found in cereals such as bread wheat (Triticum aestivum ssp. aestivum) and spelt (T. aestivum ssp. spelta). Among them, the α-gliadins display the highest immunogenicity, with four T-cell stimulatory epitopes. The toxicity of each epitope sequence can be reduced or even suppressed according to the allelic form of each sequence. One way to address the CD problem would be to make use of this allelic variability in breeding programs to develop safe varieties, but tools to track the presence of toxic epitopes are required. The objective of this study was to develop a tool to accurately detect and quantify the immunogenic content of expressed α-gliadins of spelt and bread wheat.
机译:背景乳糜泻(CD)是由谷物中发现的麸质蛋白的特定序列引起的,这些谷物如面包小麦(Triticum aestivum ssp。aestivum)和拼写(T. aestivum ssp。spelta)。其中,α-麦醇溶蛋白显示出最高的免疫原性,具有四个T细胞刺激性表位。根据每个序列的等位基因形式,可以降低或什至抑制每个表位序列的毒性。解决CD问题的一种方法是在育种计划中利用这种等位基因的变异性来开发安全的品种,但是需要使用工具来追踪毒性表位的存在。这项研究的目的是开发一种工具,以准确检测和定量拼写和面包小麦表达的α-麦醇溶蛋白的免疫原性含量。

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