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首页> 美国卫生研究院文献>The Plant Pathology Journal >Chlorophyll a Fluorescence Parameters of Hulled and Hull-less Barley (Hordeum vulgare L.) DH Lines Inoculated with Fusarium culmorum
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Chlorophyll a Fluorescence Parameters of Hulled and Hull-less Barley (Hordeum vulgare L.) DH Lines Inoculated with Fusarium culmorum

机译：枯萎镰刀菌接种去壳和去壳大麦DH系的叶绿素a荧光参数

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Barley worldwide is affected seriously by Fusarium seedling blight (FSB) and Fusarium head blight (FHB) diseases caused by the Fusarium species. The objective of this study was to facilitate the resistance of hulled and hull-less barley at different growth stages to F. culmorum according to direct parameters: disease rating (DR), fresh weight of leaves and roots, kernel weight per spike, kernel number per spike, plump kernels, and indirect parameters - chlorophyll a fluorescence (CF). Plate assay, greenhouse and field tests were performed on 30 spring barley doubled haploid (DH) lines and their parents infected with Fusarium culmorum. Direct parameters proved that hulled genotypes show less symptoms. Most studied chlorophyll a fluorescence (CF) parameters (apart from DIo/CS – amount of energy dissipated from PSII for laboratory test, TRo/CS – amount of excitation energy trapped in PSII reaction centers, ETo/CS – amount of energy used for electron transport and RC/CS – number of active reaction centres in the state of fully reduced PSII reaction center in field experiment) were significantly affected by F. culmorum infection. In all experiments, hulled genotypes had higher values of CF parameters compared to hull-less ones. Significant correlations were detected between direct and indirect parameters and also between various environments. It was revealed that ABS/CS, TRo/CS, and RC/CS have significant positive correlation in greenhouse test and field experiment. Significant correlations suggest the possibility of applying the CF parameters in selection of barley DH lines resistant to F. culmorum infection.
机译：全世界的大麦严重受到镰刀菌种引起的枯萎病和镰刀菌病的影响。这项研究的目的是根据直接参数：疾病等级（DR），叶和根的新鲜重量，每个穗的粒重，粒数，促进不同生长阶段的去壳大麦和无壳大麦对鳞茎镰刀菌的抗性。每个峰值，饱满的籽粒和间接参数-叶绿素a荧光（CF）。在30个春季大麦双单倍体（DH）品系及其亲本感染了镰刀菌后，进行了平板测定，温室和田间试验。直接参数证明脱壳的基因型显示较少的症状。研究最多的叶绿素a荧光（CF）参数（除了DIo / CS –用于实验室测试的PSII消散的能量数量，TRo / CS – PSII反应中心中捕获的激发能的数量，ETo / CS –用于电子的能量数量运输和RC / CS –在现场实验中，处于完全减少的PSII反应中心状态的活跃反应中心的数量）受到了镰刀菌感染的显着影响。在所有实验中，与无壳基因型相比，有壳基因型的CF参数值更高。在直接和间接参数之间以及各种环境之间检测到显着的相关性。结果表明，在温室试验和田间试验中，ABS / CS，TRo / CS和RC / CS具有显着的正相关。显着的相关性表明可能在选择对镰刀菌感染有抗性的大麦DH系中应用CF参数。

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