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Molecular Identification and Evaluation of Indigenous Bacterial Isolates for Their Plant Growth Promoting and Biological Control Activities against Fusarium Wilt Pathogen of Tomato

机译:番茄番茄枯萎病病原菌对植物生长的促进作用及其生物防治活性的分子鉴定与评价

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摘要

In search of an effective biological control agent against the tomato pathogen Fusarium oxysporum f. sp. lycopersici, rhizospheric soil samples were collected from eight agro-ecological zones of Bangladesh. Among the bacteria isolated from soil, 24 isolates were randomly selected and evaluated for their antagonistic activity against F. oxysporum f. sp. lycopersici. The two promising antagonistic isolates were identified as Brevundimonas olei and Bacillus methylotrophicus based on morphological, biochemical and molecular characteristics. These two isolates were evaluated for their biocontrol activity and growth promotion of two tomato cultivars (cv. Pusa Rubi and Ratan) for two consecutive years. Treatment of Pusa Rubi and Ratan seeds with B. olei prior to inoculation of pathogen caused 44.99% and 41.91% disease inhibition respectively compared to the untreated but pathogen-inoculated control plants. However, treatment of Pusa Rubi and Ratan seeds with B. methylotrophicus caused 24.99% and 39.20% disease inhibition respectively. Furthermore, both the isolates enhanced the growth of tomato plants. The study revealed that these indigenous bacterial isolates can be used as an effective biocontrol agent against Fusarium wilt of tomato.
机译:为了寻找有效的抗番茄病原菌镰刀菌f。的生物防治剂。 sp。 lycopersici,根际土壤样品采集自孟加拉国的八个农业生态区。从土壤中分离出的细菌中,随机选择了24个分离株,并评估了它们对尖孢镰刀菌的拮抗活性。 sp。 lycopersici。根据形态,生化和分子特征,鉴定出两个有希望的拮抗分离物为油酸短杆菌和甲基营养芽孢杆菌。连续两年对这两个分离株的生防活性和两个番茄品种(cusa。Pusa Rubi和Ratan)的生长促进进行了评估。与未处理但接种病原体的对照植物相比,在接种病原体之前用油菜芽胞杆菌处理Pusa Rubi和Ratan种子分别引起44.99%和41.91%的病害抑制。然而,用甲基营养芽孢杆菌处理Pusa Rubi和Ratan种子分别引起24.99%和39.20%的疾病抑制。此外,两种分离物均增强了番茄植物的生长。研究表明,这些本土细菌分离株可用作抗番茄枯萎病的有效生物防治剂。

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