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首页> 美国卫生研究院文献>Life >The Heptameric SmAP1 and SmAP2 Proteins of the Crenarchaeon Sulfolobus Solfataricus Bind to Common and Distinct RNA Targets
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The Heptameric SmAP1 and SmAP2 Proteins of the Crenarchaeon Sulfolobus Solfataricus Bind to Common and Distinct RNA Targets

机译：七节鱼Sulfolobus Solfataricus的七聚体SmAP1和SmAP2蛋白与常见和不同的RNA目标绑定。

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摘要

Sm and Sm-like proteins represent an evolutionarily conserved family with key roles in RNA metabolism. Sm-based regulation is diverse and can range in scope from eukaryotic mRNA splicing to bacterial quorum sensing, with at least one step in these processes being mediated by an RNA-associated molecular assembly built on Sm proteins. Despite the availability of several 3D-structures of Sm-like archaeal proteins (SmAPs), their function has remained elusive. The aim of this study was to shed light on the function of SmAP1 and SmAP2 of the crenarchaeon Sulfolobus solfataricus (Sso). Using co-purification followed by RNASeq different classes of non-coding RNAs and mRNAs were identified that co-purified either with both paralogues or solely with Sso-SmAP1 or Sso-SmAP2. The large number of associated intron-containing tRNAs and tRNA/rRNA modifying RNAs may suggest a role of the two Sso-SmAPs in tRNA/rRNA processing. Moreover, the 3D structure of Sso-SmAP2 was elucidated. Like Sso-SmAP1, Sso-SmAP2 forms homoheptamers. The binding of both proteins to distinct RNA substrates is discussed in terms of surface conservation, structural differences in the RNA binding sites and differences in the electrostatic surface potential of the two Sso-SmAP proteins. Taken together, this study may hint to common and different functions of both Sso-SmAPs in Sso RNA metabolism.
机译：Sm和Sm样蛋白代表在RNA代谢中起关键作用的进化保守家族。基于Sm的调控是多种多样的，范围从真核mRNA剪接到细菌群体感应，这些过程中的至少一步是由建立在Sm蛋白上的RNA相关分子组装介导的。尽管可获得Sm样古细菌蛋白（SmAP）的几种3D结构，但其功能仍然难以捉摸。这项研究的目的是阐明crenarchaeon Sulfolobus solfataricus（Sso）的SmAP1和SmAP2的功能。使用共纯化，然后进行RNASeq鉴定，可以鉴定出与旁系同源物或仅与Sso-SmAP1或Sso-SmAP2共纯化的不同类别的非编码RNA和mRNA。大量相关的含内含子的tRNA和tRNA / rRNA修饰RNA可能暗示了两种Sso-SmAP在tRNA / rRNA加工中的作用。此外，阐明了Sso-SmAP2的3D结构。像Sso-SmAP1一样，Sso-SmAP2形成同七聚体。根据表面保守性，RNA结合位点的结构差异以及两种Sso-SmAP蛋白的静电表面电势差异，讨论了两种蛋白质与不同RNA底物的结合。两者合计，这项研究可能暗示两种Sso-SmAP在Sso RNA代谢中的共同和不同功能。

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