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首页> 美国卫生研究院文献>Translational Andrology and Urology >AB219. The use of anti-VDAC2 antibody for the combined assessment of human sperm acrosome integrity and ionophore A23187-induced acrosome reaction
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AB219. The use of anti-VDAC2 antibody for the combined assessment of human sperm acrosome integrity and ionophore A23187-induced acrosome reaction

机译：AB219。抗VDAC2抗体在人精子顶体完整性和离子载体A23187诱导的顶体反应联合评估中的应用

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Voltage-dependent anion channel (VDAC) is mainly located in the mitochondrial outer membrane and participates in many biological processes. In mammals, three VDAC subtypes (VDAC1, 2 and 3) have been identified. Although VDAC has been extensively studied in various tissues and cells, there is little knowledge about the distribution and function of VDAC in male mammalian reproductive system. Several studies have demonstrated that VDAC exists in mammalian spermatozoa and is implicated in spermatogenesis, sperm maturation, motility and fertilization. However, there is no knowledge about the respective localization and function of three VDAC subtypes in human spermatozoa. In this study, we focused on the presence of VDAC2 in human spermatozoa and its possible role in the acrosomal integrity and acrosome reaction using specific anti-VDAC2 monoclonal antibody for the first time. The results exhibited that native VDAC2 existed in the membrane components of human spermatozoa. The co-incubation of spermatozoa with anti-VDAC2 antibody did not affect the acrosomal integrity and acrosome reaction, but inhibited ionophore -induced intracellular Ca increase. Our study suggested that VDAC2 was located in the acrosomal membrane or plasma membrane of human spermatozoa, and played putative roles in sperm functions through mediating Ca transmembrane transport.
机译：电压依赖性阴离子通道（VDAC）主要位于线粒体外膜中，并参与许多生物过程。在哺乳动物中，已鉴定出三种VDAC亚型（VDAC1、2和3）。尽管已经在各种组织和细胞中对VDAC进行了广泛的研究，但是关于VDAC在雄性哺乳动物生殖系统中的分布和功能的知识很少。多项研究表明，VDAC存在于哺乳动物的精子中，并与精子发生，精子成熟，运动和受精有关。但是，尚不了解人类精子中三种VDAC亚型的各自定位和功能。在这项研究中，我们重点研究了人类精子中VDAC2的存在及其首次使用特异性抗VDAC2单克隆抗体在顶体完整性和顶体反应中的可能作用。结果表明，天然VDAC2存在于人精子的膜成分中。精子与抗VDAC2抗体共同孵育不会影响顶体的完整性和顶体反应，但抑制了离子载体诱导的细胞内Ca的增加。我们的研究表明，VDAC2位于人精子的顶体膜或质膜中，并通过介导Ca跨膜转运在精子功能中发挥了推定作用。

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