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Ionic Liquid-Assisted Laser Desorption/Ionization–Mass Spectrometry: Matrices Microextraction and Separation

机译:离子液体辅助激光解吸/电离质谱:基质微萃取和分离

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摘要

Ionic liquids (ILs) have advanced a variety of applications, including matrix-assisted laser desorption/ionization–mass spectrometry (MALDI–MS). ILs can be used as matrices and solvents for analyte extraction and separation prior to analysis using laser desorption/ionization–mass spectrometry (LDI–MS). Most ILs show high stability with negligible sublimation under vacuum, provide high ionization efficiency, can be used for qualitative and quantitative analyses with and without internal standards, show high reproducibility, form homogenous spots during sampling, and offer high solvation efficiency for a wide range of analytes. Ionic liquids can be used as solvents and pseudo-stationary phases for extraction and separation of a wide range of analytes, including proteins, peptides, lipids, carbohydrates, pathogenic bacteria, and small molecules. This review article summarizes the recent advances of ILs applications using MALDI–MS. The applications of ILs as matrices, solvents, and pseudo-stationary phases, are also reviewed.
机译:离子液体(ILs)具有多种应用,包括基质辅助激光解吸/电离质谱(MALDI-MS)。在使用激光解吸/电离质谱(LDI-MS)进行分析之前,IL可用作分析物提取和分离的基质和溶剂。大多数ILs在真空下升华程度可忽略不计,显示出高稳定性,可提供高电离效率,可在有内标和无内标的情况下用于定性和定量分析,显示出高重现性,在采样过程中形成均一的斑点,并在宽范围分析物。离子液体可用作溶剂和伪平稳相,用于提取和分离各种分析物,包括蛋白质,肽,脂质,碳水化合物,病原菌和小分子。这篇综述文章总结了使用MALDI-MS进行ILs应用的最新进展。还综述了IL作为基质,溶剂和伪平稳相的应用。

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