Detection of Anammox Activity and 16S rRNA Genes in Ravine Paddy Field Soil

机译：稻田土壤厌氧氨氧化活性和16S rRNA基因的检测

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摘要

An anammox assay involving a ¹⁵N tracer and gas chromatography-mass spectrometry revealed that the potential anammox activity accounted for 1 to 5% of total N2 production in a ravine paddy field, Japan. Among four 4-cm-deep layers, the top layer showed the highest activity. Clone libraries showed that the DNA in the top layer contained sequences related to those of Candidatus ‘Brocadia fulgida’, Ca. ‘B. anammoxidans’, and Ca. ‘Kuenenia stuttgartiensis’. These results suggest that a specific population of anammox bacteria was present in paddy soils, although a small part of dinitrogen gas was emitted from the soil via anammox.

机译：包括^{15 N示踪剂和气相色谱-质谱联用的厌氧菌测定表明，潜在的厌氧菌活性占日本稻田中N2总产量的1％至5％。在四个4厘米深的层中，顶层显示出最高的活性。克隆文库显示，最上层的DNA包含与加州念珠菌“ Brocadia fulgida”的相关序列。 ‘B。 anammoxidans’和Ca。 'Kuenenia stuttgartiensis'。这些结果表明，稻田土壤中存在特定的厌氧菌细菌种群，尽管一小部分的二氧化氮气体是通过厌氧菌从土壤中排放出来的。}

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期刊名称 Microbes and Environments
作者
Yoshinori Sato; Hiroyuki Ohta; Takao Yamagishi; Yong Guo; Tomoyasu Nishizawa; M. Habibur Rahman; Hisao Kuroda; Task Kato; Masanori Saito; Ikuo Yoshinaga; Kazuyuki Inubushi; Yuichi Suwa;
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作者单位

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年(卷),期 2012(27),3
年度 2012
页码 316–319
总页数 4
原文格式 PDF
正文语种
中图分类应用微生物学;微生物学;
关键词
anaerobic ammonium oxidation ravine paddy field soil nitrogen removal;

机译：厌氧铵氧化;沟壑区土壤;脱氮;

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专利

1. Detection of anammox activity and 16S rRNA genes in ravine paddy field soil. [J] . Sato Y., Ohta H., Yamagishi T., Microbes and Environments . 2012,第3期

机译：在稻田土壤中检测厌氧氨氧化活性和16S rRNA基因。
2. Detection of Anammox Activity and 16S rRNA Genes in Ravine Paddy Field Soil [J] . Yoshinori Sato, Hiroyuki Ohta, Takao Yamagishi, Microbes and Environments . 2012,第3期

机译：稻田土壤厌氧氨氧化活性和16S rRNA基因的检测
3. Molecular analyses reveal stability of bacterial communities in bulk soil of a Japanese paddy field: estimation by denaturing gradient gel electrophoresis of 16S rRNA genes amplified from DNA accompanied with RNA [J] . Kikuchi H, Watanabe T, Jia ZhongJun, Soil Science and Plant Nutrition . 2007,第4期

机译：分子分析揭示了日本稻田土壤中细菌群落的稳定性：通过变性梯度凝胶电泳对伴随DNA扩增的DNA扩增的16S rRNA基因进行评估
4. Changes in community structure and transcriptional activity of methanogenic archaea in a paddy field soil brought about by a water-saving practice -Estimation by PCR-DGGE and qPCR of 16S rDNA and 16S [C] . Takeshi Watanabe, Yasukazu Hosen, Ruth Agbisit, World Congress of Soil Science . 2011

机译：通过PCR-DGGE和16S RDNA和16S的QPCR产生的稻田土壤中甲状原能古亚甲基甲基型甲状腺古代甲状腺古亚叶的转录活性
5. Soil community analysis along a salt gradient using denaturing gradient gel electrophoresis of PCR-amplified 16S rRNA genes. [D] . Dutta, Partha. 2006

机译：使用PCR扩增的16S rRNA基因的变性梯度凝胶电泳，沿着盐梯度进行土壤群落分析。
6. Comparative Phylogenetic Assignment of Environmental Sequences of Genes Encoding 16S rRNA and Numerically Abundant Culturable Bacteria from an Anoxic Rice Paddy Soil [O] . Ulf Hengstmann, Kuk-Jeong Chin, Peter H. Janssen, 1999

机译：缺氧水稻土中编码16S rRNA基因和数量丰富的可培养细菌的环境序列的系统发生比较分配。
7. Assessment of molecular detection of anaerobic ammonium-oxidizing (anammox) bacteria in different environmental samples using PCR primers based on 16S rRNA and functional genes [O] . Ping Han, Uli Klümper, Alex Wong, 2017

机译：基于16S rRNA和官能基因的PCR引物评估不同环境样品中不同环境样品中厌氧铵氧化（厌氧毒素）细菌的分子检测

Detection of Anammox Activity and 16S rRNA Genes in Ravine Paddy Field Soil

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