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首页> 美国卫生研究院文献>Microbes and Environments >Culture-Dependent and -Independent Identification of Polyphosphate-Accumulating Dechloromonas spp. Predominating in a Full-Scale Oxidation Ditch Wastewater Treatment Plant
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Culture-Dependent and -Independent Identification of Polyphosphate-Accumulating Dechloromonas spp. Predominating in a Full-Scale Oxidation Ditch Wastewater Treatment Plant

机译：依赖文化的和独立的聚磷酸盐累积脱氯单胞菌属的鉴定。在大型氧化沟废水处理厂中占主导地位

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The oxidation ditch process is one of the most economical approaches currently used to simultaneously remove organic carbon, nitrogen, and also phosphorus (P) from wastewater. However, limited information is available on biological P removal in this process. In the present study, microorganisms contributing to P removal in a full-scale oxidation ditch reactor were investigated using culture-dependent and -independent approaches. A microbial community analysis based on 16S rRNA gene sequencing revealed that a phylotype closely related to Dechloromonas spp. in the family Rhodocyclaceae dominated in the oxidation ditch reactor. This dominant Dechloromonas sp. was successfully isolated and subjected to fluorescent staining for polyphosphate, followed by microscopic observations and a spectrofluorometric analysis, which clearly demonstrated that the Dechloromonas isolate exhibited a strong ability to accumulate polyphosphate within its cells. These results indicate the potential key role of Dechloromonas spp. in efficient P removal in the oxidation ditch wastewater treatment process.
机译：氧化沟工艺是目前用于从废水中同时去除有机碳，氮以及磷（P）的最经济的方法之一。但是，在此过程中关于生物除磷的信息有限。在本研究中，使用培养依赖性和非依赖性方法研究了在大型氧化沟反应器中去除P的微生物。基于16S rRNA基因测序的微生物群落分析表明，系统型与Dechloromonas spp密切相关。红景天科中的一个在氧化沟反应器中占主导地位。这种占主导地位的Dechloromonas sp。分离成功后，对多磷酸盐进行了荧光染色，然后进行了显微镜观察和荧光分光光度法分析，这清楚地表明脱氯单胞菌分离物表现出很强的在其细胞内积累多磷酸盐的能力。这些结果表明了Dechloromonas spp的潜在关键作用。在氧化沟废水处理过程中有效去除磷。

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