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首页> 美国卫生研究院文献>Journal of Microbiology Biology Education >Biotechnology by Design: An Introductory Level Project-Based Synthetic Biology Laboratory Program for Undergraduate Students
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Biotechnology by Design: An Introductory Level Project-Based Synthetic Biology Laboratory Program for Undergraduate Students

机译：按设计的生物技术：面向本科生的入门级基于项目的合成生物学实验室计划

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Synthetic biology offers an ideal opportunity to promote undergraduate laboratory courses with research-style projects, immersing students in an inquiry-based program that enhances the experience of the scientific process. We designed a semester-long, project-based laboratory curriculum using synthetic biology principles to develop a novel sensory device. Students develop subject matter knowledge of molecular genetics and practical skills relevant to molecular biology, recombinant DNA techniques, and information literacy. During the spring semesters of 2014 and 2015, the Synthetic Biology Laboratory Project was delivered to sophomore genetics courses. Using a cloning strategy based on standardized BioBrick genetic “parts,” students construct a “reporter plasmid” expressing a reporter gene (GFP) controlled by a hybrid promoter regulated by the lac-repressor protein (lacI). In combination with a “sensor plasmid,” the production of the reporter phenotype is inhibited in the presence of a target environmental agent, arabinose. When arabinose is absent, constitutive GFP expression makes cells glow green. But the presence of arabinose activates a second promoter (pBAD) to produce a lac-repressor protein that will inhibit GFP production. Student learning was assessed relative to five learning objectives, using a student survey administered at the beginning (pre-survey) and end (post-survey) of the course, and an additional 15 open-ended questions from five graded Progress Report assignments collected throughout the course. Students demonstrated significant learning gains (p < 0.05) for all learning outcomes. Ninety percent of students indicated that the Synthetic Biology Laboratory Project enhanced their understanding of molecular genetics. The laboratory project is highly adaptable for both introductory and advanced courses.
机译：合成生物学提供了一个理想的机会，以研究型项目促进本科生实验课程的发展，使学生沉浸于基于探究的计划中，以增强科学过程的经验。我们使用合成生物学原理设计了一个学期的，基于项目的实验室课程，以开发一种新颖的感官设备。学生将学习分子遗传学的主题知识以及与分子生物学，重组DNA技术和信息素养相关的实践技能。在2014年和2015年春季学期，“合成生物学实验室项目”交付给了大二遗传学课程。使用基于标准化BioBrick遗传“部分”的克隆策略，学生构建了一个“报告质粒”，该质粒表达由lac阻遏蛋白（lacI）调控的杂交启动子控制的报告基因（GFP）。与“传感器质粒”组合，在目标环境因子阿拉伯糖的存在下，报告基因表型的产生受到抑制。缺少阿拉伯糖时，组成型GFP表达会使细胞发出绿色光。但是阿拉伯糖的存在会激活第二个启动子（pBAD），从而产生可抑制GFP产生的lac阻遏蛋白。通过在课程开始（调查前）和调查结束（调查后）进行的学生调查，以及从整个过程中收集的五份分级进度报告作业中另外获得的15个开放式问题，对与五个学习目标相关的学生学习进行了评估课程。学生在所有学习成果中均表现出显着的学习收获（p <0.05）。 90％的学生表示“合成生物学实验室计划”增强了他们对分子遗传学的理解。该实验室项目非常适合入门课程和高级课程。

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