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首页> 美国卫生研究院文献>Journal of Microbiology Biology Education >Undergraduates Phenotyping Arabidopsis Knockouts in a Course-Based Undergraduate Research Experience: Exploring Plant Fitness and Vigor Using Quantitative Phenotyping Methods
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Undergraduates Phenotyping Arabidopsis Knockouts in a Course-Based Undergraduate Research Experience: Exploring Plant Fitness and Vigor Using Quantitative Phenotyping Methods

机译：基于课程的本科生研究经验中的拟南芥拟南芥基因敲除：使用定量表型方法探索植物适应性和活力

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摘要

We present a curriculum description, an initial student outcome investigation, and sample scientific results for a representative Course-Based Undergraduate Research Experience (CURE) that is part of the “Undergraduates Phenotyping Arabidopsis Knockouts” (unPAK) network. CUREs in the unPAK network characterize quantitative phenotypes of the model plant Arabidopsis from across environments to uncover connections between genotype and phenotype. Students in unPAK CUREs grow plants in a replicated block design and make quantitative measurements throughout the semester. This CURE enables students to answer plant science questions that draw from fields such as environmental science, genetics, ecology, and evolution. Findings indicate that this experience provides students with opportunities to make relevant scientific discoveries. Eighty percent of student datasets produced from the CURE met criteria for inclusion in the project database, indicative of student learning in data collection and analysis of quantitative plant traits. Student datasets uncovered novel effects of mutation on plant form. In addition, students’ science self-efficacy increased as a result of course participation, and faculty feedback on course implementation was positive. We present unPAK as a new network that supports CUREs and research experiences focused on collecting biological data made publicly available to the scientific community. The unPAK CUREs can be tailored to address instructor interests or pedagogical needs while involving students in research investigating quantitative plant phenotypes.
机译：我们提供了课程描述，初步的学生成果调查，以及代表课程为基础的本科生研究经验（CURE）的科学结果样本，该经验是“本科生拟南芥基因敲除”（unPAK）网络的一部分。 unPAK网络中的CURE可以表征模型植物拟南芥在不同环境下的定量表型，从而揭示基因型和表型之间的联系。 unPAK CURE的学生采用重复模块设计种植植物，并在整个学期进行定量测量。该CURE使学生能够回答来自环境科学，遗传学，生态学和进化等领域的植物科学问题。调查结果表明，这种经历为学生提供了进行相关科学发现的机会。从CURE产生的学生数据集中，有80％符合纳入项目数据库的标准，这表明学生可以在数据收集和定量植物性状分析中学习。学生数据集揭示了突变对植物形态的新影响。此外，由于参加了课程，学生的科学自我效能得到了提高，教师对课程实施的反馈也很积极。我们提出unPAK作为支持CURE和研究经验的新网络，该研究专注于收集公开提供给科学界的生物学数据。可以定制unPAK CURE，以满足教师的兴趣或教学需求，同时让学生参与研究定量植物表型的研究。

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