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A Visualization Technique of a Unique pH Distribution around an Ion Depletion Zone in a Microchannel by Using a Dual-Excitation Ratiometric Method

机译:双通道比率法可视化微通道中离子耗竭区周围独特pH分布的可视化技术

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摘要

The ion depletion zone of ion concentration polarization has a strong potential to act as an immaterial barrier, separating delicate submicron substances, including biomolecules, without causing physical damage. However, the detailed mechanisms of the barrier effect remain incompletely understood because it is difficult to visualize the linked behavior of protons, cations, anions, and charged molecules in the thin ion depletion zone. In this study, pH distribution in an ion depletion zone was measured to estimate the role of proton behavior. This was done in order to use it as a tool with good controllability for biomolecule handling in the future. As a result, a unique pH peak was observed at several micrometers distance from the microchannel wall. The position of the peak appeared to be in agreement with the boundary of the ion depletion zone. From this agreement, it is expected that the pH peak has a causal connection to the barrier effect of the ion depletion zone.
机译:离子浓度极化的离子耗尽区具有强大的潜力,可以充当非物质屏障,在不造成物理损坏的情况下分离出包括生物分子在内的易碎的亚微米物质。但是,由于很难形象化质子,阳离子,阴离子和带电分子在薄离子耗尽区中的联系行为,因此对屏障作用的详细机制仍未完全了解。在这项研究中,测量了离子耗竭区的pH分布以估计质子行为的作用。这样做是为了将来将其用作具有良好可控性的工具来处理生物分子。结果,在距微通道壁几微米的距离处观察到独特的pH峰。峰的位置似乎与离子耗尽区的边界一致。根据该协议,可以预期pH峰与离子耗尽区的势垒效应有因果关系。

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