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Defining Cell Cluster Size by Dielectrophoretic Capture at an Array of Wireless Electrodes of Several Distinct Lengths

机译:通过介电泳捕获在几个不同长度的无线电极阵列上定义细胞簇的大小。

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摘要

Clusters of biological cells play an important role in normal and disease states, such as in the release of insulin from pancreatic islets and in the enhanced spread of cancer by clusters of circulating tumor cells. We report a method to pattern cells into clusters having sizes correlated to the dimensions of each electrode in an array of wireless bipolar electrodes (BPEs). The cells are captured by dielectrophoresis (DEP), which confers selectivity, and patterns cells without the need for physical barriers or adhesive interactions that can alter cell function. Our findings demonstrate that this approach readily achieves fine control of cell cluster size over a broader range set by other experimental parameters. These parameters include the magnitude of the voltage applied externally to drive capture at the BPE array, the rate of fluid flow, and the time allowed for DEP-based cell capture. Therefore, the reported method is anticipated to allow the influence of cluster size on cell function to be more fully investigated.
机译:生物细胞簇在正常和疾病状态中起重要作用,例如在胰岛中胰岛素的释放以及循环肿瘤细胞簇在癌症扩散中的作用增强。我们报告了一种将细胞图案化为簇的方法,该簇的大小与无线双极电极(BPEs)阵列中每个电极的尺寸相关。通过介电电泳(DEP)捕获细胞,赋予选择性,并在不需要物理屏障或可以改变细胞功能的粘附相互作用的情况下对细胞进行图案化。我们的发现表明,这种方法可以在其他实验参数设定的较宽范围内轻松实现对细胞簇大小的精细控制。这些参数包括在外部施加以驱动BPE阵列捕获的电压大小,流体流速和基于DEP的细胞捕获所需的时间。因此,预期该报道的方法可以更充分地研究簇大小对细胞功能的影响。

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