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The Usual Suspects 2019: of Chips Droplets Synthesis and Artificial Cells

机译:2019年通常的怀疑:芯片液滴合成和人造细胞

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摘要

Synthetic biology aims to understand fundamental biological processes in more detail than possible for actual living cells. Synthetic biology can combat decomposition and build-up of artificial experimental models under precisely controlled and defined environmental and biochemical conditions. Microfluidic systems can provide the tools to improve and refine existing synthetic systems because they allow control and manipulation of liquids on a micro- and nanoscale. In addition, chip-based approaches are predisposed for synthetic biology applications since they present an opportune technological toolkit capable of fully automated high throughput and content screening under low reagent consumption. This review critically highlights the latest updates in microfluidic cell-free and cell-based protein synthesis as well as the progress on chip-based artificial cells. Even though progress is slow for microfluidic synthetic biology, microfluidic systems are valuable tools for synthetic biology and may one day help to give answers to long asked questions of fundamental cell biology and life itself.
机译:合成生物学旨在比实际的活细胞更详细地了解基本生物学过程。合成生物学可以在精确控制和定义的环境和生化条件下抵抗人工实验模型的分解和积累。微流体系统可以提供改进和完善现有合成系统的工具,因为它们允许在微米和纳米级别上控制和操纵液体。另外,基于芯片的方法易于合成生物学应用,因为它们提供了一种能够在低试剂消耗下实现全自动高通量和内容筛选的合适的技术工具包。这篇评论批评性地强调了无微细胞和基于细胞的蛋白质合成的最新进展,以及基于芯片的人工细胞的进展。尽管微流体合成生物学的进展缓慢,但微流体系统是合成生物学的宝贵工具,有朝一日可能有助于解答基本细胞生物学和生命本身这一长期存在的问题。

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