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首页> 美国卫生研究院文献>Molecular and Cellular Biology >RAD7 gene of Saccharomyces cerevisiae: transcripts nucleotide sequence analysis and functional relationship between the RAD7 and RAD23 gene products.
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RAD7 gene of Saccharomyces cerevisiae: transcripts nucleotide sequence analysis and functional relationship between the RAD7 and RAD23 gene products.

机译：酿酒酵母RAD7基因：转录本核苷酸序列分析以及RAD7和RAD23基因产物之间的功能关系。

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摘要

The RAD7 gene of Saccharomyces cerevisiae was cloned on a 4.0-kilobase (kb) DNA fragment and shown to provide full complementation of a rad7-delta mutant strain. The nucleotide sequence of a 2.2-kb DNA fragment which contains the complete RAD7 gene was determined. Transcription of the RAD7 gene initiates at multiple sites in a region spanning positions -61 to -8 of the DNA sequence. The 1.8-kb RAD7 mRNA encodes a protein of 565 amino acids with a predicted size of 63.7 kilodaltons. The hydropathy profile of the RAD7 protein indicates a highly hydrophilic amino terminus and a very hydrophobic region toward the carboxyl terminus. A RAD7 subclone deleted for the first 99 codons complements the rad7-delta mutation, but not the rad7-delta rad23-delta double mutation, indicating that the RAD23 protein can compensate for the function that is missing in the amino-terminally deleted RAD7 protein. The RAD7 and RAD23 genes in multicopy plasmids do not complement the rad23-delta and rad7-delta mutations, respectively. These observations could mean that although the two proteins might share a common functional domain, they must also perform distinct functions. Alternatively, an interaction between the RAD7 and RAD23 proteins could also account for these observations.
机译：将酿酒酵母的RAD7基因克隆到一个4.0碱基（kb）的DNA片段上，并显示出它可以完全互补rad7-delta突变株。确定了包含完整RAD7基因的2.2-kb DNA片段的核苷酸序列。 RAD7基因的转录起始于跨越DNA序列-61至-8位的区域中的多个位点。 1.8 kb RAD7 mRNA编码565个氨基酸的蛋白质，预测大小为63.7千道尔顿。 RAD7蛋白的亲水性谱表明高度亲水的氨基末端和朝向羧基末端的非常疏水的区域。删除第一个99个密码子的RAD7亚克隆可补充rad7-delta突变，但不能弥补rad7-del rad23-delta双重突变，这表明RAD23蛋白可以补偿在氨基末端缺失的RAD7蛋白中缺少的功能。多拷贝质粒中的RAD7和RAD23基因分别不补充rad23-delta和rad7-delta突变。这些观察结果可能意味着尽管这两种蛋白质可能共享一个共同的功能域，但它们还必须执行不同的功能。另外，RAD7和RAD23蛋白之间的相互作用也可以解释这些现象。

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