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首页> 美国卫生研究院文献>Molecular and Cellular Biology >The mouse immunoglobulin kappa light-chain genes are located in early- and late-replicating regions of chromosome 6.
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The mouse immunoglobulin kappa light-chain genes are located in early- and late-replicating regions of chromosome 6.

机译：小鼠免疫球蛋白κ轻链基因位于6号染色体的早期和晚期复制区域。

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摘要

The murine immunoglobulin kappa (kappa) light-chain multigene family includes the constant region (C kappa), joining-region genes, and approximately 30 kappa-variable (V kappa) region families. The entire region occupies an estimated 1,000 to 3,000 kilobases, and some V kappa families have been linked by recombinant inbred mapping. The C kappa gene and 14 V kappa families replicated differently among cell lines of lymphoid and nonlymphoid origin. In nonlymphoid cells, the C kappa gene replicated earlier than the V kappa families. A transition from replication during the second third of S phase for the C kappa gene to later replication during S for V kappa families was observed. The V kappa family (V kappa 21) that maps closest to the C kappa gene, replicated during the first half of the S phase; most of the other V kappa families replicated during the second half of S, and some replicated during the last quarter of the S phase. In lymphoid cells, the kappa locus replicated earlier in the pre-B than in the B-cell lines. In one pre-B-cell line, 22D6, the kappa genes examined replicated at the beginning of the S phase. In the B-cell lines, the EcoRI segment containing the transcribed gene replicated near the beginning of the S phase. Other V kappa families replicated within the first two-thirds of S phase. Some linked V kappa families replicated at similar times. In the B-cell lines, a transition from replication at the beginning of S for the transcribed C kappa and V kappa genes and surrounding DNA sequences to later replication for the other V kappa families was observed. However, in contrast to the non-lymphoid cell lines, the replication of this locus occurred predominantly during the first half of S. The kappa locus contains both early- and late-replicating genes, and early replication is usually associated with transcriptional activity. The results are discussed with respect to the organization of transcriptionally active chromatin domains.
机译：鼠免疫球蛋白κ（κ）轻链多基因家族包括恒定区（C kappa），连接区基因和大约30个κ可变区（V kappa）。整个地区约占1,000至3,000千碱基，一些V kappa家族已通过重组近交作图法联系在一起。 Cκ基因和14 Vκ家族在淋巴和非淋巴来源的细胞系中复制不同。在非淋巴样细胞中，Cκ基因比Vκ家族更早复制。观察到从Cκ基因的S期的后三分之一的复制到Vκ家族的S期的复制的过渡。在S期前半段复制的最接近Cκ基因的Vκ家族（Vκ21）；其他大多数V kappa家族在S期的后半部分复制，而某些在S期的最后四分之一期间复制。在淋巴样细胞中，κ基因座在pre-B中的复制要早于B细胞系。在一个前B细胞系22D6中，所检查的kappa基因在S期开始时复制。在B细胞系中，含有转录基因的EcoRI片段在S期开始时复制。其他V kappa家族在S期的前三分之二内复制。一些关联的V kappa家族在相似的时间复制。在B细胞系中，观察到从转录的C kappa和V kappa基因以及周围的DNA序列在S开头的复制过渡到其他V kappa家族的后来复制。但是，与非淋巴样细胞系相反，该基因座的复制主要发生在S的前半部分。κ基因座既包含早期复制基因又包含晚期复制基因，并且早期复制通常与转录活性相关。关于转录活性染色质结构域的组织讨论了结果。

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