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首页> 美国卫生研究院文献>Molecules >Antioxidant Capacity of Pure Compounds and Complex Mixtures Evaluated by the ORAC-Pyrogallol Red Assay in the Presence of Triton X-100 Micelles
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Antioxidant Capacity of Pure Compounds and Complex Mixtures Evaluated by the ORAC-Pyrogallol Red Assay in the Presence of Triton X-100 Micelles

机译：Triton X-100胶束存在下通过ORAC-邻苯三酚红分析评估纯化合物和复杂混合物的抗氧化能力

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摘要

The protective effect of different antioxidants and complex mixtures on the consumption of pyrogallol red (PGR) induced by peroxyl radicals was studied in the absence and presence of Triton X-100 micelles. The presence of micelles decreased significantly the protection of PGR afforded by lipophilic antioxidants (β-carotene, octyl gallate), while no effect of micelles was observed for hydrophilic antioxidants such as Trolox, caffeic acid, gallic acid, and ascorbic acid. In the presence of complex mixtures a clear effect of Triton X-100 micelles was also observed in the protection afforded by wines, tea infusions, and seed extracts of Eugenia jambolana and Myrciaria cauliflora. On the other hand, no effect of micelles was observed for orange juice and pulp fruit extracts. The ORAC (Oxygen Radical Absorbance Capacity) index was evaluated in the absence (ORAC-PGR) and presence of Triton X-100 micelles (ORAC-PGRMIC). Triton X-100 micelles affect ORAC-PGR values of antioxidants in a lipophilicity-dependent way. From the obtained results, we conclude that ORAC-PGR and ORAC-PGRMIC assays could be considered as an alternative to estimate the antioxidant ability (ORAC-PGR) and to infer the association to Triton X-100 micelles (ORAC-PGR/ORAC-PGRMIC) of pure antioxidants and their complex mixtures.
机译：在Triton X-100胶束不存在和存在的情况下，研究了不同的抗氧化剂和复杂混合物对过氧自由基诱导的邻苯三酚红（PGR）消耗的保护作用。胶束的存在显着降低了亲脂性抗氧化剂（β-胡萝卜素，没食子酸辛酯）对PGR的保护，而对于亲水性抗氧化剂（如Trolox，咖啡酸，没食子酸和抗坏血酸）则没有胶束的作用。在存在复杂混合物的情况下，还可以观察到Triton X-100胶束在葡萄酒，茶叶浸入液和番木瓜和菜豆Myrciaria cauliflora的种子提取物的保护作用中具有明显的作用。另一方面，对于橙汁和果肉果实提取物没有观察到胶束的作用。在不存在（ORAC-PGR）和存在Triton X-100胶束（ORAC-PGRMIC）的情况下评估ORAC（氧自由基吸收能力）指数。 Triton X-100胶束以亲脂性依赖的方式影响抗氧化剂的ORAC-PGR值。根据获得的结果，我们得出结论，可以将ORAC-PGR和ORAC-PGRMIC分析视为评估抗氧化能力（ORAC-PGR）并推断与Triton X-100胶束（ORAC-PGR / ORAC- PGRMIC）的纯抗氧化剂及其复杂混合物。

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