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首页> 美国卫生研究院文献>Molecules >Biocatalytic Behaviour of Immobilized Rhizopus oryzae Lipase in the 13-Selective Ethanolysis of Sunflower Oil to Obtain a Biofuel Similar to Biodiesel
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Biocatalytic Behaviour of Immobilized Rhizopus oryzae Lipase in the 13-Selective Ethanolysis of Sunflower Oil to Obtain a Biofuel Similar to Biodiesel

机译：固定化米根霉脂肪酶在葵花籽油13-选择性乙醇分解中获得类似于生物柴油的生物燃料的生物催化行为

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A new biofuel similar to biodiesel was obtained in the 1,3-selective transesterification reaction of sunflower oil with ethanol using as biocatalyst a Rhizopus oryzae lipase (ROL) immobilized on Sepiolite, an inorganic support. The studied lipase was a low cost powdered enzyme preparation, Biolipase-R, from Biocon-Spain, a multipurpose additive used in food industry. In this respect, it is developed a study to optimize the immobilization procedure of these lipases on Sepiolite. Covalent immobilization was achieved by the development of an inorganic-organic hybrid linker formed by a functionalized hydrocarbon chain with a pendant benzaldehyde, bonded to the AlPO4 support surface. Thus, the covalent immobilization of lipases on amorphous AlPO4/sepiolite (20/80 wt %) support was evaluated by using two different linkers (p-hydroxybenzaldehyde and benzylamine-terephthalic aldehyde, respectively). Besides, the catalytic behavior of lipases after physical adsorption on the demineralized sepiolite  was also evaluated. Obtained results indicated that covalent immobilization with the p-hydroxybenzaldehyde linker gave the best biocatalytic behavior. Thus, this covalently immobilized lipase showed a remarkable stability as well as an excellent capacity of reutilization (more than five successive reuses) without a significant loss of its initial catalytic activity. This could allow a more efficient fabrication of biodiesel minimizing the glycerol waste production.
机译：使用固定在无机载体Sepiolite上的米根霉脂肪酶（ROL）作为生物催化剂，在葵花籽油与乙醇的1,3-选择性酯交换反应中获得了类似于生物柴油的新型生物燃料。研究的脂肪酶是一种廉价的粉状酶制剂，即Biocon-Spain的Biolipase-R，它是食品工业中使用的多用途添加剂。在这方面，已进行研究以优化这些脂肪酶在海泡石上的固定程序。共价固定化是通过开发一种无机-有机杂化连接基团来实现的，该杂化基团由官能化的烃链与侧基苯甲醛组成，并键合到AlPO4载体表面上。因此，通过使用两种不同的接头（分别为对羟基苯甲醛和苄胺对苯二甲酸醛）评估脂肪酶在无定形AlPO4 /海泡石（20/80 wt％）载体上的共价固定。此外，还评估了脱脂海泡石上物理吸附后脂肪酶的催化行为。获得的结果表明，用对羟基苯甲醛接头共价固定化具有最佳的生物催化性能。因此，这种共价固定的脂肪酶显示出显着的稳定性以及出色的再利用能力（超过五次连续再利用），而其初始催化活性没有明显损失。这可以允许更有效地制造生物柴油，从而最大程度地减少甘油废物的产生。

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