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首页> 美国卫生研究院文献>Molecules >Analysis of Flavonoids in Lotus (Nelumbo nucifera) Leaves and Their Antioxidant Activity Using Macroporous Resin Chromatography Coupled with LC-MS/MS and Antioxidant Biochemical Assays
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Analysis of Flavonoids in Lotus (Nelumbo nucifera) Leaves and Their Antioxidant Activity Using Macroporous Resin Chromatography Coupled with LC-MS/MS and Antioxidant Biochemical Assays

机译：大孔树脂色谱联用LC-MS / MS和抗氧化生化分析分析荷叶中的黄酮类化合物及其抗氧化活性

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摘要

Lotus (Nelumbo nucifera) leaves, a traditional Chinese medicinal herb, are rich in flavonoids. In an effort to thoroughly analyze their flavonoid components, macroporous resin chromatography coupled with HPLC-MS/MS was employed to simultaneously enrich and identify flavonoids from lotus leaves. Flavonoids extracted from lotus leaves were selectively enriched in the macroporous resin column, eluted subsequently as fraction II, and successively subjected to analysis with the HPLC-MS/MS and bioactivity assays. Altogether, fourteen flavonoids were identified, four of which were identified from lotus leaves for the first time, including quercetin 3-O-rhamnopyranosyl-(1→2)-glucopyranoside, quercetin 3-O-arabinoside, diosmetin 7-O-hexose, and isorhamnetin 3-O-arabino- pyranosyl-(1→2)-glucopyranoside. Further bioactivity assays revealed that these flavonoids from lotus leaves possess strong antioxidant activity, and demonstrate very good potential to be explored as food supplements or even pharmaceutical products to improve human health.
机译：荷叶（Nelumbo nucifera）是一种传统的中草药，富含黄酮类化合物。为了全面分析其类黄酮成分，采用了大孔树脂色谱结合HPLC-MS / MS来同时富集和鉴定荷叶中的类黄酮。从荷叶中提取的黄酮类化合物在大孔树脂柱中选择性富集，随后以级分II洗脱，并依次进行HPLC-MS / MS分析和生物活性测定。总共鉴定出14种黄酮类化合物，其中首次从荷叶中鉴定出了4种黄酮类化合物，包括槲皮素3-O-鼠李吡喃糖基-（1→2）-吡喃葡萄糖苷，槲皮素3-O-阿拉伯糖苷，薯os皂素7-O-己糖，异佛瑞汀3-O-阿拉伯糖基-吡喃糖基-（1→2）-吡喃葡萄糖苷。进一步的生物活性测定表明，荷叶中的这些黄酮类化合物具有很强的抗氧化活性，并显示出非常好的潜力，可以作为食品补充剂甚至药品来改善人体健康。

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