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首页> 美国卫生研究院文献>Molecules >Rapid Estimation of Tocopherol Content in Linseed and Sunflower Oils-Reactivity and Assay
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Rapid Estimation of Tocopherol Content in Linseed and Sunflower Oils-Reactivity and Assay

机译：亚麻籽和葵花籽油中生育酚含量的快速估算-反应性和含量测定

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摘要

The reactivity of tocopherols with 2,2-diphenyl-1-picrylhydrazyl (DPPH) was studied in model systems in order to establish a method for quantifying vitamin E in plant oils. The method was optimized with respect to solvent composition of the assay medium, which has a large influence on the course of reaction of tocopherols with DPPH. The rate of reaction of α-tocopherol with DPPH is higher than that of γ-tocopherol in both protic and aprotic solvents. In ethyl acetate, routinely applied for the analysis of antioxidant potential (AOP) of plant oils, reactions of tocopherols with DPPH are slower and concentration of tocopherols in the assay has a large influence on their molar reactivity. In 2-propanol, however, two electrons are exchanged for both α- and γ-tocopherols, independent of their concentration. 2-propanol is not toxic and is fully compatible with polypropylene labware. The chromatographically determined content of tocopherols and their molar reactivity in the DPPH assay reveal that only tocopherols contribute to the AOP of sunflower oil, whereas the contribution of tocopherols to the AOP of linseed oil is 75%. The DPPH assay in 2-propanol can be applied for rapid and cheap estimation of vitamin E content in plant oils where tocopherols are major antioxidants.
机译：在模型系统中研究了生育酚与2,2-二苯基-1-吡啶并肼基（DPPH）的反应性，从而建立了定量植物油中维生素E的方法。该方法针对测定介质的溶剂组成进行了优化，这对生育酚与DPPH的反应过程具有很大的影响。在质子和非质子溶剂中，α-生育酚与DPPH的反应速率均高于γ-生育酚。在通常用于分析植物油抗氧化能力（AOP）的乙酸乙酯中，生育酚与DPPH的反应较慢，并且生育酚在测定中的浓度对其摩尔反应性有很大影响。然而，在2-丙醇中，α-和γ-生育酚交换两个电子，而与它们的浓度无关。 2-丙醇无毒，与聚丙烯实验室器具完全兼容。色谱测定的生育酚含量及其在DPPH分析中的摩尔反应性表明，只有生育酚有助于葵花籽油的AOP，而生育酚对亚麻籽油AOP的贡献为75％。 2-生育酚中的DPPH分析可用于快速廉价地估算生育酚为主要抗氧化剂的植物油中维生素E的含量。

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