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Comparative Evaluation of Chemical Profiles of Pyrrosiae Folium Originating from Three Pyrrosia Species by HPLC-DAD Combined with Multivariate Statistical Analysis

机译：HPLC-DAD结合多元统计分析比较评价三种火生菌的火棘叶化学特征

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摘要

Pyrrosiae Folium (PF) is a commonly used Chinese herb medicine originating from three Pyrrosia species for the treatment of urinary infection and urolithiasis. According to Chinese medicine practice, different specie origins led to some variations in the therapeutic effects of PF. To ensure the safety and efficacy of PF in clinical practice, it is necessary to establish a reliable and integrative method to distinguish PF occurring from the three species. In the present paper, a HPLC–DAD method was developed and applied to simultaneously analyze five major compounds in PF. Afterwards, multivariate statistical analyses including principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were applied for specie discrimination and integrative quality evaluation based on quantitative data. The chemical determination and pattern recognition results of 35 batches of PF samples indicated that PF samples from three species showed different chemical profiles and could be discriminated clearly. In conclusion, the present method is rapid and reliable for the quality assessment and species discrimination of PF.
机译：黄热病叶（PF）是一种常见的中草药，起源于3个热病物种，用于治疗尿路感染和尿石症。根据中医实践，不同物种的起源导致PF的治疗效果有所不同。为了确保PF在临床上的安全性和有效性，有必要建立一种可靠的综合方法来区分这3种物种中发生的PF。本文开发了一种HPLC-DAD方法，并将其用于同时分析PF中的五种主要化合物。此后，将包括主成分分析（PCA）和偏最小二乘判别分析（PLS-DA）在内的多元统计分析用于基于数量数据的物种鉴别和综合质量评估。 35批次PF样品的化学测定和模式识别结果表明，三种物种的PF样品显示出不同的化学特征，可以清晰地区分。综上所述，本方法对PF的质量评估和物种鉴别是快速，可靠的。

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