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首页> 美国卫生研究院文献>Molecules >Preparation and Evaluation of Oseltamivir Molecularly Imprinted Polymer Silica Gel as Liquid Chromatography Stationary Phase
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Preparation and Evaluation of Oseltamivir Molecularly Imprinted Polymer Silica Gel as Liquid Chromatography Stationary Phase

机译：奥司他韦分子印迹聚合物硅胶液相色谱固定相的制备与评价

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摘要

To improve the chromatographic performance of an oseltamivir (OS) molecularly imprinted polymer (MIP), silica gel coated with an MIP layer for OS (OSMIP@silica gel) was prepared by the surface molecular imprinting technology on the supporter of porous silica gel microspheres. A nonimprinted polymer with the silica gel (NIP@silica gel) was also prepared for comparison. The obtained particles were characterized through FT–IR, scanning electron microscopy, specific surface area analysis, and porosity measurements. The results indicated that the polymer was successfully synthesized and revealed the structural differences between imprinted and nonimprinted polymers. The results of static adsorption experiments showed that adsorption quantity of the OSMIP@silica gel for OS was higher than that for NIP@silica gel, and the OSMIP@silica gel had two kinds of affinity sites for OS but the NIP@silica gel had one. The chromatographic performance of the OSMIP@silica gel column had significant improvement. The imprinting factor of the OSMIP@silica gel column for OS was 1.64. Furthermore, the OSMIP@silica gel column showed good affinity and selectivity for template OS and another neuraminidase inhibitor, peramivir, but not for quinocetone. These results indicated that the prepared OSMIP could be used to simulate the activity center of neuraminidase, and the OSMIP@silica gel column could be also employed in future studies to search for more active neuraminidase inhibitor analogues from traditional Chinese herbs.
机译：为了提高奥司他韦（OS）分子印迹聚合物（MIP）的色谱性能，通过表面分子印迹技术在多孔硅胶微球的载体上制备了涂有OS的MIP层的硅胶（OSMIP @硅胶）。还制备了具有硅胶的非压印聚合物（NIP @硅胶）用于比较。通过FT-IR，扫描电子显微镜，比表面积分析和孔隙率测量对获得的颗粒进行表征。结果表明该聚合物已成功合成，并揭示了印迹和非印迹聚合物之间的结构差异。静态吸附实验结果表明，OSMIP @硅胶对OS的吸附量高于NIP @硅胶，并且OSMIP @硅胶对OS具有两种亲和力位点，而NIP @硅胶具有一个。 OSMIP @硅胶柱的色谱性能有了显着提高。 OSMIP @硅胶柱对OS的印迹因子为1.64。此外，OSMIP @硅胶色谱柱对模板OS和另一种神经氨酸酶抑制剂培拉米韦表现出良好的亲和力和选择性，但对喹烯酮却没有。这些结果表明，所制备的OSMIP可用于模拟神经氨酸酶的活性中心，OSMIP @硅胶柱还可用于未来的研究中，以从中草药中寻找更具活性的神经氨酸酶抑制剂类似物。

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