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首页> 美国卫生研究院文献>Molecules >In Vivo and In Vitro Anti-Arthritic Effects of Cardenolide-Rich and Caffeoylquinic Acid-Rich Fractions of Periploca forrestii
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In Vivo and In Vitro Anti-Arthritic Effects of Cardenolide-Rich and Caffeoylquinic Acid-Rich Fractions of Periploca forrestii

机译：百里香中富胡烯基内酯和富含咖啡酰奎尼酸的馏分的体内和体外抗节育作用

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摘要

Periploca forrestii Schltr. (P. forrestii) is a species used in Traditional Chinese Medicine (TCM) known as “Miao medicine”, and has a long history of use in the treatment of rheumatism, rheumatoid arthritis (RA), and joint pain. The present study aimed to evaluate the anti-arthritis effects of the cardenolide-rich and caffeoylquinic acid-rich fractions (CDLFs and CQAFs) of P. forrestii in collagen-induced arthritic (CIA) rats, and defined the mechanisms of therapeutic action in MH7A cells treated with TNF-α. Serum rheumatoid factor (RF), TNF-α, IL-6, IL-1β, PGE2, NO, SOD, and MDA were determined by ELISA or other commercially assay kits. Histopathological changes in ankle joint tissues were examined. The mRNA expressions of IL-1β, IL-6, COX-2, and iNOS in MH7A cells were measured by qRT-PCR assays. In addition, the expressions of iNOS, COX-2, and p65 proteins, and the phosphorylation of IκBα, p38, ERK1/2, and JNK proteins in MH7A cells were analyzed by Western blot. The results showed that CDLF and CQAF could suppress the paw swelling in CIA rats at different doses (125 mg/kg, 250 mg/kg, and 500 mg/kg). Histopathological examination suggests that the CDLF and CQAF significantly relieved the damage of the structure of the ankle joint in CIA rats. In addition, serum RF, TNF-α, IL-6, IL-1β, PGE2, NO, and MDA were decreased, along with increased activity of serum SOD. Furthermore, CDLF and CQAF downregulated the expressions of IL-1β, IL-6, COX-2, iNOS, and p65, and inhibited the phosphorylation of IκBα, p38, ERK1/2, and JNK in MH7A cells treated with TNF-α. These findings demonstrated that both CDLF and CQAF exhibited anti-arthritic activity, which might be associated with their inhibitory effects on the NF-κB and MAPK signaling pathways.
机译：Periploca forrestii Schltr。 （P. forrestii）是在中医药（TCM）中被称为“苗药”的物种，在治疗风湿病，类风湿关节炎（RA）和关节痛方面已有悠久的历史。本研究旨在评估在胶原诱导的关节炎（CIA）大鼠中福氏疟原虫的富含烯醇内酯和富含咖啡酰奎尼酸的馏分（CDLF和CQAFs）的抗关节炎作用，并确定了MH7A的治疗作用机制TNF-α处理的细胞。血清类风湿因子（RF），TNF-α，IL-6，IL-1β，PGE2，NO，SOD和MDA通过ELISA或其他商用测定试剂盒进行测定。检查踝关节组织的组织病理学变化。用qRT-PCR法检测MH7A细胞中IL-1β，IL-6，COX-2和iNOS的mRNA表达。此外，通过蛋白质印迹法分析了MH7A细胞中iNOS，COX-2和p65蛋白的表达以及IκBα，p38，ERK1 / 2和JNK蛋白的磷酸化。结果表明，不同剂量（125 mg / kg，250 mg / kg和500 mg / kg）的CDLF和CQAF可以抑制CIA大鼠的足爪肿胀。组织病理学检查表明，CDLF和CQAF可明显减轻CIA大鼠踝关节结构的损伤。此外，血清RF，TNF-α，IL-6，IL-1β，PGE2，NO和MDA降低，同时血清SOD活性增加。此外，CDLF和CQAF下调了TNF-α处理的MH7A细胞中IL-1β，IL-6，COX-2，iNOS和p65的表达，并抑制了IκBα，p38，ERK1 / 2和JNK的磷酸化。这些发现表明，CDLF和CQAF均显示出抗关节炎活性，这可能与其对NF-κB和MAPK信号通路的抑制作用有关。

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