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Combination of Sensory Chromatographic and Chemometrics Analysis of Volatile Organic Compounds for the Discrimination of Authentic and Unauthentic Harumanis Mangoes

机译:挥发性有机化合物的感官色谱和化学计量学分析相结合用于区分正宗和非正宗的Harumanis芒果

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摘要

This study analyzed the volatile organic compounds (VOCs) of three mango varieties (Harumanis, Tong Dam and Susu) for the discrimination of authentic Harumanis from other mangoes. The VOCs of these mangoes were extracted and analysed nondestructively using Head Space-Solid Phase Micro Extraction (HS-SPME) coupled to Gas Chromatography-Mass Spectrometry (GC-MS). Prior to the analytical method, two simple sensory analyses were carried out to assess the ability of the consumers to differentiate between the Harumanis and Tong Dam mangoes as well as their preferences towards these mangoes. On the other hand, chemometrics techniques, such as principal components analysis (PCA), hierarchical clustering analysis (HCA), and discriminant analysis (DA), were used to visualise grouping tendencies of the volatile compounds detected. These techniques were successful in identifying the grouping tendencies of the mango samples according to the presence of their respective volatile compounds, thus enabling the identification of the groups of substances responsible for the discrimination between the authentic and unauthentic Harumanis mangoes. In addition, three ocimene compounds, namely beta-ocimene, trans beta-ocimene, and allo-ocimene, can be considered as chemical markers of the Harumanis mango, as these compounds exist in all Harumanis mango, regardless the different sources of the mangoes obtained.
机译:这项研究分析了三个芒果品种(Harumanis,Tong Dam和Susu)的挥发性有机化合物(VOC),以区分正宗的Harumanis与其他芒果。使用顶空固相微萃取(HS-SPME)和气相色谱-质谱(GC-MS)进行无损提取和分析这些芒果的VOC。在采用分析方法之前,先进行了两次简单的感官分析,以评估消费者区分Harumanis和Tong Dam芒果的能力以及他们对这些芒果的偏爱。另一方面,化学计量学技术(例如主成分分析(PCA),层次聚类分析(HCA)和判别分析(DA))用于可视化检测到的挥发性化合物的分组趋势。这些技术已成功地根据其各自的挥发性化合物的存在确定了芒果样品的分组趋势,从而能够鉴定出负责鉴别真假和真假芒果的物质组。另外,可以将三种甜菜碱化合物,即β-甜菜碱,反式β-甜菜碱和异源甜菜碱,视为Harumanis芒果的化学标记物,因为这些化合物存在于所有Harumanis芒果中,而与获得的芒果来源不同无关。

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