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首页> 美国卫生研究院文献>Molecules >In Vitro and In Vivo Antioxidant Activities of the Flowers and Leaves from Paeonia rockii and Identification of Their Antioxidant Constituents by UHPLC-ESI-HRMSn via Pre-Column DPPH Reaction
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In Vitro and In Vivo Antioxidant Activities of the Flowers and Leaves from Paeonia rockii and Identification of Their Antioxidant Constituents by UHPLC-ESI-HRMSn via Pre-Column DPPH Reaction

机译：Pa药花朵和叶子的体外和体内抗氧化活性及通过柱前DPPH反应通过UHPLC-ESI-HRMSn鉴定其抗氧化成分

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The genus Paeonia, also known as the “King of Flowers” in China, is an important source of traditional Chinese medicine (TCM). Plants of this genus have been used to treat a range of cardiovascular and gynecological diseases. However, the potential pharmacological activity of one particular species, Paeonia rockii, has not been fully investigated. In the first part of the present study, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic) acid (ABTS), reducing power assays, and metal ion chelating assays were used to investigate the in vitro antioxidant activities of Paeonia rockii. In the second portion of the study, a mouse model of d-galactose-induced aging was used to validate the antioxidant effects of the flowers from Paeonia rockii in vivo. Lastly, potential antioxidant constituents were screened and identified by ultra-high pressure liquid chromatography and electrospray ionization coupled with high-resolution mass spectrometry (UHPLC-ESI-HRMSn) combined with the DPPH assay. Results indicated that the flowers and leaves exhibited stronger antioxidant activity than ascorbic acid in vitro. The therapeutic effect of Paeonia rockii was determined in relation to the levels of biochemical indicators, such as 8-iso-prostaglandin F2α (8-iso PGF2α) in the serum, superoxide dismutase (SOD), protein carbonyl, malondialdehyde (MDA), and glutathione (GSH) in the liver and brain, after daily intra-gastric administration of different concentrations of extracts (100, 200 and 400 mg/kg) for three weeks. The levels of 8-iso PGF2α (p < 0.01) and protein carbonyl groups (p < 0.01) were significantly reduced, whereas those of SOD (p < 0.05) had significantly increased, indicating that components of the flowers of Paeonia rockii had favorable antioxidant activities in vivo. Furthermore, UHPLC-ESI-HRMSn, combined with pre-column DPPH reaction, detected 25 potential antioxidant compounds. Of these, 18 compounds were tentatively identified, including 11 flavonoids, four phenolic acids, two tannins, and one monoterpene glycoside. This study concluded that the leaves and flowers from Paeonia rockii possess excellent antioxidant properties, highlighting their candidacy as “new” antioxidants, which can be utilized therapeutically to protect the body from diseases caused by oxidative stress.
机译：e药属，在中国也被称为“花中之王”，是中药的重要来源。该属植物已被用于治疗一系列心血管和妇科疾病。但是，尚未对一种特定物种species药Paeonia rockii的潜在药理活性进行充分研究。在本研究的第一部分中，使用2,2-二苯基-1-吡啶并肼基（DPPH），2,2'-叠氮基双-（3-乙基苯并噻唑啉-6-磺酸）酸（ABTS），还原能力测定法和金属离子螯合法用于研究rock药的体外抗氧化活性。在研究的第二部分中，使用d-半乳糖诱导的衰老小鼠模型验证了Pa药花的体内抗氧化作用。最后，通过超高压液相色谱和电喷雾电离结合高分辨质谱法（UHPLC-ESI-HRMS n ）结合DPPH分析法筛选和鉴定了潜在的抗氧化剂成分。结果表明，花和叶在体外显示出比抗坏血酸更强的抗氧化活性。相对于生化指标，例如血清中的8-异前列腺素F2α（8-异PGF2α），超氧化物歧化酶（SOD），蛋白质羰基，丙二醛（MDA）和每天在胃内施用不同浓度的提取物（100、200和400 mg / kg）三周后，肝脏和大脑中的谷胱甘肽（GSH）。 8-isoPGF2α（p <0.01）和蛋白质羰基（p <0.01）的含量显着降低，而SOD（p <0.05）的含量显着升高，表明rock药花的成分具有良好的抗氧化剂体内活动。此外，UHPLC-ESI-HRMS n 结合柱前DPPH反应，检测到25种潜在的抗氧化剂化合物。在这些化合物中，初步鉴定出18种化合物，包括11种类黄酮，4种酚酸，2种单宁和1种单萜糖苷。这项研究得出的结论是，Pa药的叶子和花朵具有出色的抗氧化性能，突显了它们作为“新”抗氧化剂的候选资格，可在治疗上用于保护人体免受氧化应激引起的疾病的侵害。

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