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A Novel Dicyanoisophorone-Based Ratiometric Fluorescent Probe for Selective Detection of Cysteine and Its Bioimaging Application in Living Cells

机译：一种基于双氰基异佛尔酮的比例荧光探针选择性检测半胱氨酸及其在活细胞中的生物成像应用

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摘要

A selective and ratiometric turn-on fluorescent probe was designed and synthesized by using a novel dicyanoisophorone-based derivative and acrylate moiety. The probe displayed high stability and good selectivity to cysteine (Cys) over homocysteine (Hcy) and glutathione (GSH). It also exhibited rapid response to Cys within 180 s. Most importantly, the fluorescence intensity ratio at 590 and 525 nm (I590/I525) was linearly dependent on the Cys concentration in the range from 0 to 40 μM and the detection limit calculated to be 0.48 μM. This probe was also applied for bioimaging of intracellular Cys in living HeLa cells with low cytotoxicity.

机译：通过使用新型的基于双氰基异佛尔酮的衍生物和丙烯酸酯部分，设计并合成了选择性和比例开启荧光探针。与高半胱氨酸（Hcy）和谷胱甘肽（GSH）相比，该探针显示出高稳定性和对半胱氨酸（Cys）的良好选择性。它还在180 s内对Cys表现出快速反应。最重要的是，590和525 nm处的荧光强度比（I590 / I525）与Cys浓度线性相关，范围为0至40μM，计算出的检出限为0.48μM。该探针还用于细胞毒性低的活HeLa细胞中细胞内Cys的生物成像。

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期刊名称 Molecules
作者
Hengrui Zhang; Nan Qin; Zhijie Fang;
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作者单位

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年(卷),期 2018(23),2
年度 2018
页码 475
总页数 9
原文格式 PDF
正文语种
中图分类分子生物学;
关键词
fluorescent probe dicyanoisophorone cysteine ratiometric;

机译：荧光探针;二氰基异佛尔酮;半胱氨酸;比例;

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专利

1. A fluorescent turn-on probe for highly selective detection of cysteine and its bioimaging applications in living cells and tissues [J] . Xia Lili, Zhao Ying, Huang Jinxin, Sensors and Actuators . 2018,第OCTa期

机译：一种用于高选择性检测半胱氨酸的荧光开启探针及其在活细胞和组织中的生物成像应用
2. A novel ratiometric fluorescent probe for selective detection of Hg~(2+), Cr~(3+) and Al~(3+) and its bioimaging application in living cells [J] . Yang Yang, Yan Feng, Ying-Zhe Wang, Sensors and Actuators . 2017,第deca期

机译：一种选择性检测Hg〜（2 +），Cr〜（3+）和Al〜（3+）的新型比例荧光探针及其在活细胞中的生物成像应用
3. A colorimetric and ratiometric fluorescent probe with enhanced near-infrared fluorescence for selective detection of cysteine and its application in living cells [J] . Feng Shumin, Fang Yang, Feng Weiyong, Dyes and Pigments . 2017,第期

机译：具有增强的近红外荧光的比色和比例荧光探针，用于选择性检测半胱氨酸及其在活细胞中的应用
4. N-Acetyl-L-cysteine-templated formation of the fluorescent silver nanocluster and its application to bioimaging [C] . Xiaoqing Xu, Lu Ga International Conference on Functional Materials and Nanotechnology . 2012

机译：N-乙酰基-1-半胱氨酸模板形成荧光银纳米粉丝的形成及其在生物模拟中的应用
5. Ratiometric Near-Infrared Fluorescent Probes for the Sensitive Detection of Intracellular pH and Bio-Thiols in Live Cells [D] . Xia, Shuai . 2020

机译：大率近红外荧光荧光探针，用于活细胞中细胞内pH和生物硫醇的敏感性检测
6. A Near-Infrared Ratiometric Fluorescent Probe for Highly Selective Recognition and Bioimaging of Cysteine [O] . Xuan Zhang, Li Zhang, Wei-Wei Ma, 1970

机译：半胱氨酸的高选择性识别和生物成像的近红外比率荧光探针。
7. A highly selective ratiometric fluorescent probe for the cascade detection of Zn2+ and H2PO4− and its application in living cell imaging [O] . Kui Du, Shizhen Niu, Li Qiao, 2017

机译：一种高精度的比例荧光探针，用于Zn2 +和H2PO4的级联检测及其在活细胞成像中的应用

A Novel Dicyanoisophorone-Based Ratiometric Fluorescent Probe for Selective Detection of Cysteine and Its Bioimaging Application in Living Cells

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