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首页> 美国卫生研究院文献>Molecules >Total Alkaloids from Bamboo Shoots and Bamboo Shoot Shells of Pleioblastus amarus (Keng) Keng f. and Their Anti-Inflammatory Activities
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Total Alkaloids from Bamboo Shoots and Bamboo Shoot Shells of Pleioblastus amarus (Keng) Keng f. and Their Anti-Inflammatory Activities

机译：苦竹（Pleioblastus amarus（Keng）Keng）笋和笋壳中的总生物碱。及其抗炎活性

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摘要

The bamboo shoot of Pleioblastus amarus (Keng) Keng f. is a medicinal and edible plant product in China. In this study, the chemical composition of the total alkaloids from bamboo shoots and bamboo shoot shells of P. amarus (Keng) Keng f. (ABSP and ABSSP, respectively) were separated and investigated by UHPLC/QTOF-MS/MS. The results showed that a total of 32 alkaloids were extracted, with 15 common to both ABSP and ABSSP and 10 and 7 alkaloids distinct to ABSP and ABSSP, respectively. ABSP and ABSSP both decreased the lipopolysaccharide (LPS, 0.5 μg/mL)-induced nitric oxide (NO) production in RAW264.7 murine macrophages with half maximal inhibitory concentration (IC50) values of 78 and 55 μg/mL, respectively. We also found that ABSP and ABSSP (100 μg/mL) could decrease the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) at both mRNA and protein levels in LPS-exposed RAW264.7 cells. Moreover, 100 μg/mL of ABSP and ABSSP also significantly inhibited LPS-induced mRNA expression of interleukin 1β (IL-1β) and tumor necrosis factor α (TNF-α). Additionally, ABSP and ABSSP (100 μg/mL) decreased the phosphorylation of extracellular regulated protein kinase (ERK) in LPS-stimulated RAW264.7 cells. Collectively, the total alkaloids from the bamboo shoots and shells of P. amarus exhibit anti-inflammatory effects in LPS-activated RAW264.7 cells through the inhibition of ERK signaling. This result can provide support for the medicinal use and further study of P. amarus.
机译：苦竹（Pleioblastus amarus（Keng）Keng f。是中国的药用和食用植物产品。在这项研究中，竹笋和竹笋壳中总生物碱的化学成分。分离（分别为ABSP和ABSSP）并通过UHPLC / QTOF-MS / MS进行研究。结果表明，共提取了32种生物碱，其中ABSP和ABSSP共有15种生物碱，ABSP和ABSSP分别有10种和7种生物碱。 ABSP和ABSSP均能降低RAW264.7鼠巨噬细胞中脂多糖（LPS，0.5μg/ mL）诱导的一氧化氮（NO）生成，最大抑制浓度（IC50）值分别为78和55μg/ mL。我们还发现，在暴露于LPS的RAW264.7细胞中，在mRNA和蛋白水平上，ABSP和ABSSP（100μg/ mL）均可降低诱导型一氧化氮合酶（iNOS）和环氧合酶-2（COX-2）的表达。此外，100μg/ mL的ABSP和ABSSP也显着抑制LPS诱导的白介素1β（IL-1β）和肿瘤坏死因子α（TNF-α）的mRNA表达。此外，ABSP和ABSSP（100μg/ mL）降低了LPS刺激的RAW264.7细胞中细胞外调节蛋白激酶（ERK）的磷酸化。总体而言，紫茎泽兰的竹笋和贝壳中的总生物碱通过抑制ERK信号转导而在LPS激活的RAW264.7细胞中表现出抗炎作用。该结果可为药用体育和进一步研究提供支持。

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