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首页> 美国卫生研究院文献>Molecules >Optimization of Ultrasonic-Assisted Extraction and Purification of Zeaxanthin and Lutein in Corn Gluten Meal
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Optimization of Ultrasonic-Assisted Extraction and Purification of Zeaxanthin and Lutein in Corn Gluten Meal

机译：超声辅助提取玉米面粕中玉米黄质和叶黄素的工艺研究

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摘要

Zeaxanthin and lutein have a wide range of pharmacological applications. In this study, we conducted systematic experimental research to optimize antioxidant extraction based on detection, extraction, process amplification, and purification. An ultrasonic-assisted method was used to extract zeaxanthin and lutein with high efficiency from corn gluten meal. Firstly, the effects of solid-liquid ratio, extraction temperature, and ultrasonic extraction time on the extraction of zeaxanthin were investigated in single-factor experiments. The optimization extraction parameters of zeaxanthin and lutein with ethanol solvent were obtained using the response surface methodology (RSM) as follows: liquid–solid ratio of 7.9:1, extraction temperature of 56 °C, and extraction time of 45 min. The total content of zeaxanthin and lutein was 0.501%. The optimum extraction experimental parameters were verified by process amplification, and we confirmed that the parameters of the extraction process optimized using the RSM design are reliable and precise. Zeaxanthin and lutein from crude extract of corn gluten were separated and purified using silica gel column chromatography with the purity of zeaxanthin increasing from 0.28% to 31.5% (about 110 times) and lutein from 0.25% to 16.3% (about 65 times), which could be used for large-scale industrial production of carotenoids.
机译：玉米黄质和叶黄素具有广泛的药理应用。在这项研究中，我们进行了系统的实验研究，以基于检测，提取，工艺扩增和纯化来优化抗氧化剂的提取。超声辅助法从玉米蛋白粉中高效提取玉米黄质和叶黄素。首先，在单因素实验中研究了固液比，提取温度和超声提取时间对玉米黄质提取的影响。使用响应表面方法（RSM）获得玉米黄质和叶黄素在乙醇溶剂中的最佳提取参数如下：液固比7.9：1，提取温度56°C，提取时间45分钟。玉米黄质和叶黄素的总含量为0.501％。通过工艺放大验证了最佳提取实验参数，我们确认了使用RSM设计优化的提取工艺参数是可靠且精确的。用硅胶柱色谱法分离并纯化玉米面筋粗提物中的玉米黄质和叶黄素，玉米黄质的纯度从0.28％增至31.5％（约110倍），叶黄素从0.25％增至16.3％（约65倍），可用于类胡萝卜素的大规模工业生产。

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