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Authentication of Apis cerana Honey and Apis mellifera Honey Based on Major Royal Jelly Protein 2 Gene

机译：基于蜂王浆主要蛋白2基因的蜜蜂和蜜蜂的鉴定

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摘要

In Asia, honey is mainly produced by Apis mellifera and Apis cerana. However, the price of A. cerana honey is usually much higher than A. mellifera honey. Seeing considerable profits, some dishonest companies and beekeepers mislabel A. mellifera honey as A. cerana honey or incorporate A. mellifera honey into A. cerana honey. In the present study, we developed methods to discriminate A. cerana honey from A. mellifera honey based on the MRJP2 (major royal jelly protein 2) gene. Two pairs of species-specific primers were designed. The amplification products of A. cerana and A. mellifera were 212 and 560 bp, respectively. As little as one percent incorporation of A. mellifera honey in the mixture can be detected by duplex PCR. Additionally, another method based on the melt curve analysis using the same primers was also developed, allowing a rapid discrimination of real-time PCR product of different species. Our study shows that the entomological authentication of honey samples can be identified by nuclear genes other than mitochondrial genes and this extends the possibility of gene selection in identification. The authentication system we proposed could be a useful tool for discriminating A. cerana honey from A. mellifera honey.
机译：在亚洲，蜂蜜主要由蜜蜂蜜蜂和蜜蜂蜜蜂生产。但是，cera cerana蜂蜜的价格通常比mellifera蜂蜜高得多。看到可观的利润，一些不诚实的公司和养蜂人将A. mellifera蜂蜜误贴为A. cerana蜂蜜，或将A. mellifera蜂蜜掺入了A. cerana蜂蜜中。在本研究中，我们开发了基于MRJP2（主要蜂王浆蛋白2）基因从蜜蜂A. mellifera蜂蜜中区分cer。cerana蜂蜜的方法。设计了两对物种特异性引物。中华曲霉和蜜蜂拟南芥的扩增产物分别为212和560 bp。通过双重PCR可以检测到仅将A. mellifera蜂蜜掺入混合物中。此外，还开发了另一种基于使用相同引物进行熔解曲线分析的方法，从而可以快速区分不同物种的实时PCR产物。我们的研究表明，蜂蜜样品的昆虫学鉴定可以通过线粒体基因以外的其他核基因进行鉴定，这扩展了鉴定中基因选择的可能性。我们提出的身份验证系统可能是一个有用的工具，可将A. cerana honey和A. mellifera honey区别开来。

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