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Potential of Epicoccum purpurascens Strain 5615 AUMC as a Biocontrol Agent of Pythium irregulare Root Rot in Three Leguminous Plants

机译:紫草5616 AUMC菌株在三种豆科植物中作为不规则腐霉根腐病生物防治剂的潜力

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摘要

Epicoccum purpurascens stain 5615 AUMC was investigated for its biocontrol activity against root rot disease caused by Pythium irregulare. E. purpurascens greenhouse pathogenicity tests using three leguminous plants indicated that the fungus was nonpathogenic under the test conditions. The germination rate of the three species of legume seeds treated with a E. purpurascens homogenate increased significantly compared with the seeds infested with P. irregulare. No root rot symptoms were observed on seeds treated with E. purpurascens, and seedlings appeared more vigorous when compared with the non-treated control. A significant increase in seedling growth parameters (seedling length and fresh and dry weights) was observed in seedlings treated with E. purpurascens compared to pathogen-treated seedlings. Pre-treating the seeds with the bioagent fungus was more efficient for protecting seeds against the root rot disease caused by P. irregulare than waiting for disease dispersal before intervention. To determine whether E. purpurascens produced known anti-fungal compounds, an acetone extract of the fungus was analyzed by gas chromatography mass spectrometry. The extract revealed a high percentage of the cinnamic acid derivative (trimethylsiloxy) cinnamic acid methyl ester. The E. purpurascens isolate grew more rapidly than the P. irregulare pathogen in a dual culture on potato dextrose agar nutrient medium, although the two fungi grew similarly when cultured separately. This result may indicate antagonism via antibiosis or competition.
机译:研究了Epicoccum purpurascens染料5615 AUMC对由不规则腐霉引起的根腐病的生物防治活性。使用三种豆科植物的紫癜大肠杆菌温室致病性测试表明,在测试条件下,真菌无致病性。与被不规则疟原虫侵染的种子相比,用紫癜大肠杆菌匀浆处理的三种豆类种子的发芽率显着提高。与未经处理的对照相比,在用紫癜大肠杆菌处理的种子上未观察到根腐病症状,并且幼苗显得更有活力。与用病原体处理的幼苗相比,在用紫癜大肠杆菌处理的幼苗中观察到幼苗生长参数(幼苗长度以及鲜重和干重)的显着增加。用生物制剂真菌预处理种子比在干预之前等待病害传播更为有效,以保护种子免受不规则假单胞菌引起的根腐病的侵害。为了确定紫癜大肠杆菌是否产生已知的抗真菌化合物,通过气相色谱质谱法分析了真菌的丙酮提取物。提取物显示出高百分比的肉桂酸衍生物(三甲基甲硅烷氧基)肉桂酸甲酯。在马铃薯葡萄糖琼脂营养培养基上的双重培养中,紫癜大肠杆菌的分离物比不规则变形杆菌病原菌生长更快,尽管两种真菌单独培养时生长相似。该结果可能表明通过抗生素或竞争的拮抗作用。

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