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首页> 美国卫生研究院文献>Nanomaterials >Electrochemical Characterization of Graphene and MWCNT Screen-Printed Electrodes Modified with AuNPs for Laccase Biosensor Development
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Electrochemical Characterization of Graphene and MWCNT Screen-Printed Electrodes Modified with AuNPs for Laccase Biosensor Development

机译：用于漆酶生物传感器开发的AuNPs修饰的石墨烯和MWCNT丝网印刷电极的电化学表征

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The aim of this work is to show how the integration of gold nanoparticles (AuNPs) into multi-wall-carbon-nanotubes (MWCNTs) based screen-printed electrodes and into graphene-based screen-printed electrodes (GPHs) could represent a potential way to further enhance the electrochemical properties of those electrodes based on nanoparticles. Laccase from Trametes versicolor (TvL) was immobilized over MWCNTs and GPH previously modified with AuNPs (of 5 and 10 nm). The characterization of the modified electrode surface has been carried out by cyclic voltammetry. The results showed that the use of AuNPs for modification of both graphene and MWCNTs screen-printed electrode surfaces would increase the electrochemical performances of the electrodes. MWCNTs showed better results than GPH in terms of higher electroactive area formation after modification with AuNPs. The two modified nanostructured electrodes were successively proven to efficiently immobilize the TvL; the electrochemical sensing properties of the GPH- and MWCNT-based AuNPs-TvL biosensors were investigated by choosing 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic-acid diammonium salt (ABTS), catechol and caffeic acid as laccase mediators; and the kinetic parameters of the laccase biosensor were carefully evaluated.
机译：这项工作的目的是展示如何将金纳米颗粒（AuNPs）集成到基于多壁碳纳米管（MWCNTs）的丝网印刷电极和基于石墨烯的丝网印刷电极（GPHs）中，这可能是一种潜在的方法。以进一步增强这些基于纳米粒子的电极的电化学性能。将来自Trametes versicolor（TvL）的漆酶固定在MWCNT和预先用AuNPs（5和10 nm）修饰的GPH上。修饰的电极表面的表征已通过循环伏安法进行。结果表明，使用AuNPs修饰石墨烯和MWCNTs丝网印刷的电极表面可提高电极的电化学性能。在用AuNPs修饰之后，更高的电活性区域形成方面，MWCNT显示出比GPH更好的结果。两个修饰的纳米结构电极已被成功证明可以有效地固定TvL。选择2,2'-叠氮基双（3-乙基苯并噻唑啉-6-磺酸二铵盐（ABTS），儿茶酚和咖啡酸作为漆酶，研究了基于GPH和MWCNT的AuNPs-TvL生物传感器的电化学传感特性介体；并仔细评估漆酶生物传感器的动力学参数。

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