• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Nanomaterials >Endocytosis of Corn Oil-Caseinate Emulsions In Vitro: Impacts of Droplet Sizes
【2h】

Endocytosis of Corn Oil-Caseinate Emulsions In Vitro: Impacts of Droplet Sizes

机译：玉米油酪蛋白乳液的内吞作用：液滴大小的影响

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The relative uptake and mechanisms of lipid-based emulsions of three different particle diameters by Caco-2 cells were studied. The corn oil-sodium caseinate emulsions showed little or no cytotoxicity even at 2 mg/mL protein concentration for any of the three droplet size emulsions. Confocal laser scanning microscopy (CLSM) of Nile red containing emulsions showed that the lipid-based emulsions were absorbed by Caco-2 cells. A negative correlation between the mean droplet size and cellular uptake was observed. There was a time-dependent and energy-dependent uptake as shown by incubation at different times and treatment with sodium azide a general inhibitor of active transport. The endocytosis of lipid-based emulsions was size-dependent. The internalization of nanoemulsion droplets into Caco-2 cells mainly occurred through clathrin- and caveolae/lipid raft-related pathways, while macropinocytosis route played the most important role for 556 nm emulsion endocytosis as shown by the use of specific pathway inhibitors. Permeability of the emulsion through the apical or basal routes also suggested that active transport may be the main route for lipid-based nanoemulsions. The results may assist in the design and application of lipid-based nanoemulsions in nutraceuticals and pharmaceuticals delivery.
机译：研究了三种不同粒径的脂质基乳液对Caco-2细胞的相对吸收及其机理。对于三种液滴大小的乳剂中的任何一种，即使在2 mg / mL的蛋白质浓度下，玉米油-酪蛋白酸钠乳剂也几乎没有细胞毒性。含有尼罗红的乳剂的共聚焦激光扫描显微镜（CLSM）显示，基于脂质的乳剂被Caco-2细胞吸收。观察到平均液滴大小与细胞摄取之间呈负相关。如在不同时间温育并用叠氮化钠（一种活性转运的一般抑制剂）处理所显示的，存在时间依赖性和能量依赖性摄取。基于脂质的乳液的内吞作用是尺寸依赖性的。纳米乳液液滴进入Caco-2细胞的内在化主要是通过网格蛋白和小窝/脂质筏相关途径发生的，而巨胞饮作用对556 nm乳液内吞作用起着最重要的作用，如特定途径抑制剂的使用所示。乳剂通过顶部或基础途径的渗透性还表明，主动转运可能是基于脂质的纳米乳剂的主要途径。结果可能有助于脂质类纳米乳剂在营养保健品和药物输送中的设计和应用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号