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On the mechanism of oligonucleotide-primed RNA synthesis. II. Synthesis of specific primer-initiated RNA copies suitable for DNA sequence analysis.

机译：关于寡核苷酸引发的RNA合成的机制。二。适用于DNA序列分析的特定引物引发的RNA副本的合成。

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The effect of temperature and primer concentration on oligonucleotide-primed transcription has been studied using the separated strands of a well-defined natural DNA as template. Results were similar to those obtained in the homopolymer-directed model systems. At high temperature and excess primer concentration mainly primer-initiated RNA copies are synthesized. Omission of one ribonucleoside triphosphate also makes the termination specific. The unique RNA fragments thus obtained have been used to determine the perfectly-repeated sequence of 68 base pairs in this DNA.

机译：温度和引物浓度对寡核苷酸引发的转录的影响已使用明确定义的天然DNA的分离链作为模板进行了研究。结果类似于在均聚物定向模型系统中获得的结果。在高温和过量引物浓度下，主要合成了引物引发的RNA拷贝。省略一个核糖核苷三磷酸也使终止特异性。由此获得的独特RNA片段已被用于确定该DNA中68个碱基对的完美重复序列。

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期刊名称 Nucleic Acids Research
作者
C F Van Kreijl; R H Beelen; P Borst;
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年(卷),期 1977(4),2
年度 1977
页码 445–455
总页数 11
原文格式 PDF
正文语种
中图分类分子生物学;
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1. On the mechanism of oligonucleotide-primed RNA synthesis. II. Synthesis of specific primer-initiated RNA copies suitable for DNA sequence analysis [J] . C.F. Van Kreijl, P. Borst, R.H.J. Beelen Nucleic acids research . 1977,第2期

机译：关于寡核苷酸引发的RNA合成的机制。二。合成适用于DNA序列分析的特定引物引发的RNA副本
2. Structural mechanisms of RNA recognition: sequence-specific and non-specific RNA-binding proteins and the Cas9-RNA-DNA complex [J] . Ban Ting, Zhu Jian-Kang, Melcher Karsten, Cellular and molecular life sciences: CMLS . 2015,第6期

机译：RNA识别的结构机制：序列特异性和非特异性RNA结合蛋白以及Cas9-RNA-DNA复合体
3. On the mechanism of oligonucleotide-primed RNA synthesis.I. Model studies with deoxy-homopolymer templates and Escherichia coli RNA polymerase [J] . C.F. Van Kreijl, P. Borst, R.H.J. Beelen Nucleic acids research . 1977,第2期

机译：寡核苷酸引发的RNA合成机理的研究I.用脱氧均聚物模板和大肠杆菌RNA聚合酶进行模型研究
4. Label-Free Detection of Specific RNA Sequences by a DNA-Based CMOS BioMEMS [C] . Lorena Tedeschi, Claudio Domenici, Vincenzo Russino, AISEM national conference on sensors and microsystems . 2014

机译：基于DNA的CMOS BioMEMS无标签检测特定RNA序列
5. I. Synthesis of saturated, DNA, and RNA spirocyclic-4,4-nonane nucleosides. II. Studies toward epoxy carbonate formation and the synthesis of suitable precursors. III. Methodological investigations involving the reactions of diazomethane with di-, tri-, and tetraketones. IV. Towards the total synthesis of salicifoline. [D] . Hartung, Ryan Eugene. 2005

机译：I.饱和，DNA和RNA螺环4，4-壬烷核苷的合成。二。研究环氧碳酸酯的形成和合适的前体的合成。三，方法学研究涉及重氮甲烷与二，三和四酮的反应。 IV。迈向水杨酸总合成。
6. On the mechanism of oligonucleotide-primed RNA synthesis. I. Model studies with deoxyhomopolymer templates and Escherichia coli RNA polymerase. [O] . C F Van Kreijl, R H Beelen, P Borst 1977

机译：关于寡核苷酸引发的RNA合成的机制。 I.用脱氧均聚物模板和大肠杆菌RNA聚合酶进行模型研究。
7. On the mechanism of oligonucleotide-primed RNA synthesis. II. Synthesis of specific primer-initiated RNA copies suitable for DNA sequence analysis. [O] . Van Kreijl, C F, Beelen, R H, Borst, P 1977

机译：关于寡核苷酸引发的RNA合成的机制。二。适用于DNA序列分析的特定引物引发的RNA副本的合成。

On the mechanism of oligonucleotide-primed RNA synthesis. II. Synthesis of specific primer-initiated RNA copies suitable for DNA sequence analysis.

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