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Peptides at the tRNA binding site of the crystallizable monomeric form of E. coli methionyl-tRNA synthetase.

机译：大肠杆菌甲硫氨酰-tRNA合成酶的可结晶单体形式的tRNA结合位点处的肽。

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摘要

A protein affinity labeling derivative of E. coli tRNA(fMet) carrying lysine-reactive cross-linking groups has been covalently coupled to monomeric trypsin-modified E. coli methionyl-tRNA synthetase. The cross-linked tRNA-synthetase complex has been isolated by gel filtration, digested with trypsin, and the tRNA-bound peptides separated from the bulk of the free tryptic peptides by anion exchange chromatography. The bound peptides were released from the tRNA by cleavage of the disulfide bond of the cross-linker and purified by reverse-phase high-pressure liquid chromatography, yielding three major peptides. These peptides were found to cochromatograph with three peptides of known sequence previously cross-linked to native methionyl-tRNA synthetase through lysine residues 402, 439 and 465. These results show that identical lysine residues are in close proximity to tRNA(fMet) bound to native dimeric methionyl-tRNA synthetase and to the crystallizable monomeric form of the enzyme, and indicate that cross-linking to the dimeric protein occurs on the occupied subunit of the 1:1 tRNA-synthetase complex.

机译：带有赖氨酸反应性交联基团的大肠杆菌tRNA（fMet）的蛋白质亲和标记衍生物已与单体胰蛋白酶修饰的大肠杆菌甲硫酰-tRNA合成酶共价偶联。已通过凝胶过滤分离了交联的tRNA合成酶复合物，用胰蛋白酶消化，并且通过阴离子交换色谱将tRNA结合的肽与大部分游离胰蛋白酶的肽分离。通过切割交联剂的二硫键从tRNA释放结合的肽，并通过反相高压液相色谱法纯化，得到三种主要的肽。发现这些肽与先前通过赖氨酸残基402、439和465与天然蛋氨酸-tRNA合成酶交联的三个已知序列的肽共色谱。这些结果表明，相同的赖氨酸残基与结合至天然的tRNA（fMet）紧密接近。二聚甲硫酰基-tRNA合成酶和该酶的可结晶单体形式，并表明与二聚体蛋白的交联发生在1：1 tRNA-合成酶复合物的占据的亚基上。

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期刊名称 Nucleic Acids Research
作者
L H Schulman; H Pelka; O Leon;
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作者单位

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年(卷),期 1987(15),24
年度 1987
页码 10523–10530
总页数 8
原文格式 PDF
正文语种
中图分类分子生物学;
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专利

1. Peptides at the tRNA binding site of the crystallizable monomeric form of E.coli methionyl-tRNA synthetase [J] . Heike Pelka, LaDonne H. Schulman, Oscar Leon Nucleic acids research . 1987,第24期

机译：大肠杆菌蛋氨酸-tRNA合成酶可结晶单体形式的tRNA结合位点处的肽
2. Conformational change of Escherichia coli initiator methionyl-tRNA~(fMet) upon binding to methionyl-tRNA formyl transferase [J] . Christine Mayer, Uttam L. RajBhandary Nucleic Acids Research . 2002,第13期

机译：与甲硫氨酰-tRNA甲酰基转移酶结合后，大肠杆菌启动子甲硫氨酰-tRNA〜（fMet）的构象变化
3. Use of analogues of methionine and methionyl adenylate to sample conformational changes during catalysis in Escherichia coli methionyl-tRNA synthetase. [J] . Crepin T, Schmitt E, Mechulam Y, Journal of Molecular Biology . 2003,第1期

机译：甲硫氨酸和甲硫氨酸腺苷酸的类似物在大肠杆菌甲硫氨酸-tRNA合成酶催化过程中的样品构象变化中的应用。
4. Genome-wide identification of TSS, promoters and transcriptional factor binding sites in E. coli K-12 bacterial genome: Development of a bioinformatics tool for the graphical display of the high throughput sequencing data [C] . Enrique Morett, Leticia Vega-Alvarado, Ryosuke L.A.Watanabe, The 7th Asia-Pacific Bioinformatics Conference（第七届亚太生物信息学大会） . 2009

机译：大肠杆菌K-12细菌基因组中TSS，启动子和转录因子结合位点的全基因组鉴定：开发用于以图形方式显示高通量测序数据的生物信息学工具
5. Characterization of the editing activity and tRNA recognition in Escherichia coli leucine tRNA synthetase. [D] . Williams, Amy Marie. 2007

机译：大肠杆菌亮氨酸tRNA合成酶中编辑活性和tRNA识别的表征。
6. Significance of the third tRNA binding site the E site on E. coli ribosomes for the accuracy of translation: an occupied E site prevents the binding of non-cognate aminoacyl-tRNA to the A site. [O] . U Geigenmüller, K H Nierhaus 1990

机译：大肠杆菌核糖体上第三个tRNA结合位点E位点对于翻译准确性的意义：占据的E位点可防止非同源氨基酰基tRNA与A位点结合。
7. Peptides at the tRNA binding site of the crystallizable monomeric form of E. coli methionyl-tRNA synthetase. [O] . Schulman, L H, Pelka, H, Leon, O 1987

机译：大肠杆菌甲硫氨酰-tRNA合成酶的可结晶单体形式的tRNA结合位点处的肽。

Peptides at the tRNA binding site of the crystallizable monomeric form of E. coli methionyl-tRNA synthetase.

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