首页> 美国卫生研究院文献>Nucleic Acids Research >Acoustical investigation of poly(dA).poly(dT) polyd(A-T) poly(A).poly(U) and DNA hydration in dilute aqueous solutions.
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Acoustical investigation of poly(dA).poly(dT) polyd(A-T) poly(A).poly(U) and DNA hydration in dilute aqueous solutions.

机译:稀水溶液中聚(dA)。聚(dT)聚d(A-T)聚(A)。聚(U)和DNA水化的声学研究。

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摘要

Apparent molar adiabatic compressibilities and apparent molar volumes of poly[d(A-T)].poly[d(A-T)], poly(dA).poly(dT), DNA and poly(A).poly(U) in aqueous solutions were determined at 1 degree C. The change of concentration increment of the ultrasonic velocity upon replacing counter ion Cs+ by the Mg2+ ion was also determined for these polymers. The following conclusions have been made: (1) the hydration of the double helix of poly(dA).poly(dT) is remarkably larger than that of other polynucleotides; (2) the hydration of the AT pair in the B-form DNA is larger than that of the GC pair; (3) the substitution of Cs+ for Mg2+ ions as counter ions results in a decrease of hydration of the system polynucleotide plus Mg2+, and (4) the magnitude of this dehydration depends on the nucleotide sequence; the following rule is true: the lesser is a polynucleotide hydration, the larger dehydration upon changing Cs+ for Mg2+ ions in the ionic atmosphere of polynucleotide.
机译:水溶液中的poly [d(AT)]。poly [d(AT)],poly(dA).poly(dT),DNA和poly(A).poly(U)的表观摩尔绝热可压缩性和表观摩尔体积为这些聚合物还测定了在用Mg2 +离子代替抗衡离子Cs +时超声速度的浓度增量变化。得出以下结论:(1)poly(dA).poly(dT)双螺旋的水合明显大于其他多核苷酸的水合; (2)B型DNA中AT对的水合作用大于GC对。 (3)用Cs +替代Mg2 +离子作为抗衡离子,导致系统多核苷酸与Mg2 +的水合减少,并且(4)脱水的程度取决于核苷酸序列;以下规则是正确的:多核苷酸的水合越少,在多核苷酸的离子气氛中改变Ms2 +离子的Cs +时,脱水越大。

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