首页> 美国卫生研究院文献>Nucleic Acids Research >Telomeric repeat amplification without shortening or lengthening of the telomerase products: a method to analyze the processivity of telomerase enzyme

【2h】

Telomeric repeat amplification without shortening or lengthening of the telomerase products: a method to analyze the processivity of telomerase enzyme

机译：端粒重复扩增不会缩短或延长端粒酶产物：端粒酶的生产力分析方法

代理获取

本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。

页面导航

摘要
著录项
相似文献
相关主题

摘要

The Telomeric Repeat Amplification Protocol (TRAP) and its modified versions (including ours, TP-TRAP) change the size and/or the ratio of the telomerase products in the amplification stage of the assay. Based on our recently published method we developed a new TRAP. This method ensures that the number of telomeric repeats present in the original telomerase products does not change on PCR amplification. The usefulness of the method was proved with amplification of chemically synthesized telomerase products and a newly designed telomerase substrate oligonucleotide. This is the first report in which the PCR products directly reflect the size distribution of telomerase products generated by the enzyme.

机译：端粒重复扩增协议（TRAP）及其修改版本（包括我们的TP-TRAP）会在测定的扩增阶段改变端粒酶产物的大小和/或比例。基于我们最近发布的方法，我们开发了一种新的TRAP。该方法确保了原始端粒酶产物中存在的端粒重复序列的数量在PCR扩增时不会改变。通过扩增化学合成的端粒酶产物和新设计的端粒酶底物寡核苷酸证明了该方法的有效性。这是第一份报告，其中PCR产物直接反映了该酶产生的端粒酶产物的大小分布。

著录项

期刊名称 Nucleic Acids Research
作者
Istvan Szatmari; Janos Aradi;
展开▼
作者单位

展开▼
年(卷),期 2001(29),2
年度 2001
页码 e3
总页数 4
原文格式 PDF
正文语种
中图分类分子生物学;
关键词

相似文献

外文文献
中文文献
专利

1. Telomeric repeat amplification, without shortening or lengthening of the telomerase products: a method to analyze the processivity of telomerase enzyme. [J] . Szatmari I, Aradi J Nucleic Acids Research . 2001,第2期

机译：端粒重复扩增，而不会缩短或延长端粒酶产物：一种分析端粒酶合成能力的方法。
2. Telomeric repeat amplification, without shortening or lengthening of the telomerase products: a method to analyze the processivity of telomerase enzyme [J] . Istvan Szatmari, Janos Aradi Nucleic acids research . 2001,第2期

机译：端粒重复扩增，而不会缩短或延长端粒酶产物：一种分析端粒酶合成能力的方法
3. Real-time telomeric repeat amplification protocol using the duplex scorpion and two reverse primers system: The high sensitive and accurate method for quantification of telomerase activity. [J] . Huang YP, Liu ZS, Tang H, Clinica chimica acta: International journal of clinical chemistry and applied molecular biology . 2006,第1a2期

机译：使用双工蝎子和两个反向引物系统的实时端粒重复扩增方案：定量端粒酶活性的高灵敏度和准确方法。
4. The role of telomere shortening in somatic stem cells and tissue aging: lessons from telomerase model systems [C] . Stefan Tümpel, K. Lenhard Rudolph International Conference and Workshop on Hematopoietic Stem Cells . 2012

机译：端粒缩短在体细胞干细胞和组织衰老中的作用：来自端粒酶模型系统的课程
5. Exploring the telomeric repeat addition processivity of vertebrate telomerase. [D] . Xie, Mingyi. 2010

机译：探索脊椎动物端粒酶的端粒重复添加持续性。
6. Gel staining methods for detection of telomerase activity with the telomeric repeat amplification protocol (TRAP) assay. [O] . M Fujita, S Tomita, Y Ueda, 1998

机译：端粒重复扩增协议（TRAP）分析用于检测端粒酶活性的凝胶染色方法。
7. Telomeric repeat amplification, without shortening or lengthening of the telomerase products: a method to analyze the processivity of telomerase enzyme [O] . Szatmári, István, Aradi, János 2017

机译：不缩短或延长端粒酶产物的端粒重复扩增：分析端粒酶的持续合成能力的方法

Telomeric repeat amplification without shortening or lengthening of the telomerase products: a method to analyze the processivity of telomerase enzyme

摘要

著录项

相似文献

相关主题

期刊订阅