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首页> 美国卫生研究院文献>Nucleic Acids Research >The complete nucleotide sequence and RNA editing content of the mitochondrial genome of rapeseed (Brassica napus L.): comparative analysis of the mitochondrial genomes of rapeseed and Arabidopsis thaliana
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The complete nucleotide sequence and RNA editing content of the mitochondrial genome of rapeseed (Brassica napus L.): comparative analysis of the mitochondrial genomes of rapeseed and Arabidopsis thaliana

机译：油菜（Brassica napus L.）线粒体基因组的完整核苷酸序列和RNA编辑内容：油菜和拟南芥线粒体基因组的比较分析

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The entire mitochondrial genome of rapeseed (Brassica napus L.) was sequenced and compared with that of Arabidopsis thaliana. The 221 853 bp genome contains 34 protein-coding genes, three rRNA genes and 17 tRNA genes. This gene content is almost identical to that of Arabidopsis. However the rps14 gene, which is a pseudo-gene in Arabidopsis, is intact in rapeseed. On the other hand, five tRNA genes are missing in rapeseed compared to Arabidopsis, although the set of mitochondrially encoded tRNA species is identical in the two Cruciferae. RNA editing events were systematically investigated on the basis of the sequence of the rapeseed mitochondrial genome. A total of 427 C to U conversions were identified in ORFs, which is nearly identical to the number in Arabidopsis (441 sites). The gene sequences and intron structures are mostly conserved (more than 99% similarity for protein-coding regions); however, only 358 editing sites (83% of total editings) are shared by rapeseed and Arabidopsis. Non-coding regions are mostly divergent between the two plants. One-third (about 78.7 kb) and two-thirds (about 223.8 kb) of the rapeseed and Arabidopsis mitochondrial genomes, respectively, cannot be aligned with each other and most of these regions do not show any homology to sequences registered in the DNA databases. The results of the comparative analysis between the rapeseed and Arabidopsis mitochondrial genomes suggest that higher plant mitochondria are extremely conservative with respect to coding sequences and somewhat conservative with respect to RNA editing, but that non-coding parts of plant mitochondrial DNA are extraordinarily dynamic with respect to structural changes, sequence acquisition and/or sequence loss.
机译：对油菜（Brassica napus L.）的整个线粒体基因组进行测序，并与拟南芥进行比较。 221 853 bp的基因组包含34个蛋白质编码基因，3个rRNA基因和17个tRNA基因。该基因含量与拟南芥几乎相同。但是，rps14基因是拟南芥属的伪基因，在油菜籽中是完整的。另一方面，尽管拟南芥中两个线粒体中线粒体编码的tRNA种类相同，但与油菜籽相比，油菜中缺少五个tRNA基因。基于油菜线粒体基因组序列，系统地研究了RNA编辑事件。在ORF中总共鉴定出427 C到U的转化，这与拟南芥中的441个位点几乎相同。基因序列和内含子结构大部分是保守的（蛋白质编码区的相似度超过99％）；但是，油菜籽和拟南芥只有358个编辑位点（占全部编辑位点的83％）。非编码区在两种植物之间大部分是不同的。油菜和拟南芥线粒体基因组分别有三分之一（约78.7 kb）和三分之二（约223.8 kb）无法相互比对，这些区域中的大多数与DNA数据库中注册的序列没有任何同源性。油菜籽和拟南芥线粒体基因组之间的比较分析结果表明，高等植物线粒体在编码序列方面极为保守，而在RNA编辑方面则有些保守，但是植物线粒体DNA的非编码部分相对于动态而言异常动态结构变化，序列获取和/或序列丢失。

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