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首页> 美国卫生研究院文献>Journal of Virology >Control of protein synthesis by a temperature-sensitive mutant of reovirus 3. I. Temperature-sensitive function of ts261-b mutant.
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Control of protein synthesis by a temperature-sensitive mutant of reovirus 3. I. Temperature-sensitive function of ts261-b mutant.

机译：呼肠孤病毒3的温度敏感性突变体控制蛋白质合成。I. ts261-b突变体的温度敏感性功能。

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摘要

The ability of a temperature-sensitive (ts) mutant of reovirus, ts261-b, to synthesize virus-specific RNAs and proteins during infection at the nonpermissive temperature (37 degrees C) was investigated. The relative amounts of the mutant virus-specific single-stranded (ss) RNA's and double-stranded (ds) RNA's synthesized in cells at 37 degrees C were 20 to 25% as much as those synthesized in the wild-type virus-infected cells. The 10 segments of the mutant ds RNAs and the three size classes of the ss RNAs were synthesized in the usual proportions. The methylation of the mutant viral mRNA's (ss RNAs) was not blocked at 37 degrees C in infected cells. A striking temperature-sensitive restricted function of the ts261-b mutant was expressed in the synthesis of the viral proteins. This study, which uses an in vitro protein-synthesizing system reconstituted with an endogenous polysomal fraction and a postribosomal supernatant from reovirus-infected cells, has demonstrated that the endogenous polysomes obtained from ts261-b mutant-infected cells at 37 degrees C are not active in the synthesis of the viral polypeptides of known molecular weights, and the amounts of the mutant viral polypeptides synthesized in vitro by these polysomes are 5 to 9% of those synthesized by the corresponding fraction from wild-type-infected cells. The impaired protein-synthesizing capacity of the mutant virus-specific polysomes can be restored during maintenance of the infected cells at 30 degrees C after shift-down from 37 degrees C. The in vitro synthesis of viral polypeptides of known size by the active endogenous polysomes derived from cells infected at the permissive temperature is accelerated by the addition of the postribosomal supernatant obtained from cells infected at the permissive temperature. The postribosomal supernatant from mutant-infected cells at 37 degrees C did not have a stimulatory effect, but rather, it inhibited in vitro viral protein synthesis.
机译：研究了呼肠孤病毒的温度敏感（ts）突变体ts261-b在非容许温度（37摄氏度）感染期间合成病毒特异性RNA和蛋白质的能力。在37摄氏度的细胞中合成的突变病毒特异性单链（ss）RNA和双链（ds）RNA的相对量是在野生型病毒感染的细胞中合成的相对量的20％至25％ 。突变ds RNA的10个片段和ss RNA的三个大小类别按常规比例合成。在感染细胞中，突变病毒mRNA（ss RNA）的甲基化在37°C时未被阻断。在病毒蛋白的合成中表达了ts261-b突变体的显着的温度敏感性限制性功能。这项研究使用体外蛋白合成系统，该系统用呼肠孤病毒感染细胞的内源性多体部分和核糖体后上清液重构，已证明在37摄氏度下从ts261-b突变体感染的细胞中获得的内源性多核糖体没有活性在合成已知分子量的病毒多肽时，这些多核糖体在体外合成的突变病毒多肽的量是由野生型感染细胞相应级分合成的突变病毒多肽的5-9％。从37摄氏度下移后，在将感染细胞维持在30摄氏度的过程中，可以恢复突变病毒特异性多核糖体受损的蛋白合成能力。通过添加从在许可温度下感染的细胞获得的核糖体后上清液，可以加速在许可温度下感染的细胞产生的核糖核酸。来自突变感染细胞的37摄氏度的核糖体后上清液没有刺激作用，但它抑制了体外病毒蛋白的合成。

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