• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Journal of Virology >Macromolecular Synthesis in Cells Infected by Frog Virus 3 XII. Viral Regulatory Proteins in Transcriptional and Post-Transcriptional Controls
【2h】

Macromolecular Synthesis in Cells Infected by Frog Virus 3 XII. Viral Regulatory Proteins in Transcriptional and Post-Transcriptional Controls

机译：青蛙病毒3 XII感染的细胞中的大分子合成。转录和转录后控制中的病毒调节蛋白。

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Using fluorophenylalanine (FPA) to interfere with functional viral protein synthesis, we have investigated the complex transcriptional and post-transcriptional controls that operate in cells infected with frog virus 3. Our previous data, obtained by polyacrylamide gel electrophoresis of viral RNAs and proteins, showed that the addition of FPA at the beginning of infection completely prevented the synthesis of late viral RNAs and late viral proteins and blocked the normal progressive decline in the rates of synthesis of two quantitatively different classes (class I and class II) of early proteins. These results indicated that the initiation of late RNA and late protein syntheses, as well as the post-transcriptional regulation of early protein synthesis, was under the control of virus-specific proteins (D. B. Willis, R. Goorha, M. Miles, and A. Granoff, J. Virol. >24:326-342, 1977). In this communication, we show that the viral protein required to “turn on” the synthesis of late RNAs and late (class III) proteins was made within 1 to 1.5 h postinfection (p.i.); when we added FPA after this time, we observed the synthesis of all of the late macromolecules. The data also suggest that another viral protein, separate from the “turn-on” protein, controlled the abundance of late RNAs. In addition, at least two separate proteins were involved in the post-transcriptional regulation of two classes of early proteins. When FPA addition was delayed until 2 h p.i., the rate of synthesis of class I proteins (which normally peaked at 2 h p.i.) was reduced by 6 h p.i. just as in a normal infection, but the rate of synthesis of class II proteins (which normally reached a maximum at 4 h p.i. before declining) was reduced only when we waited until 3 or 4 h p.i. to add FPA. These experiments corroborate and extend previous evidence for the existence of numerous viral regulatory proteins in the control of frog virus 3 gene expression at the transcriptional and post-transcriptional levels.
机译：使用氟苯丙氨酸（FPA）干扰功能性病毒蛋白质合成，我们研究了在青蛙病毒3感染的细胞中起作用的复杂转录和转录后控制。我们以前的数据（通过聚丙烯酰胺凝胶电泳获得的病毒RNA和蛋白质）显示在感染开始时添加FPA完全阻止了晚期病毒RNA和晚期病毒蛋白的合成，并阻止了两种定量上不同的类别（I类和II类）早期蛋白质的合成速率正常逐渐下降。这些结果表明，晚期RNA和晚期蛋白质合成的起始以及早期蛋白质合成的转录后调控受病毒特异性蛋白质的控制（DB Willis，R。Goorha，M。Miles和A Granoff，J.Virol。> 24 ：326-342，1977年。在这种交流中，我们表明“开启”晚期RNA和晚期（III类）蛋白合成所需的病毒蛋白是在感染后1至1.5小时内制成的（p.i.）；当我们在此时间之后添加FPA时，我们观察到了所有晚期大分子的合成。数据还表明，与“开启”蛋白不同的另一种病毒蛋白控制着晚期RNA的丰度。此外，至少两种不同的蛋白质参与了两类早期蛋白质的转录后调控。当FPA的添加延迟到p.i. 2 h时，I类蛋白的合成速率（通常在2 h p.i达到峰值）降低了6 hp.i。就像在正常感染中一样，但是只有当我们等到3或4 h p.i时，II类蛋白质的合成速率（通常在p.i下降4 h才达到最大值）才降低。添加FPA。这些实验证实并扩展了在转录和转录后水平上控制青蛙病毒3基因表达的大量病毒调节蛋白的存在的证据。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号